The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
The periplasmic coiled coil formed by the assembly platform proteins PulL and PulM is critical for function of the Klebsiella type II secretion system
Open accessPublished version of a preprint
Abstract
Bacteria use type II secretion systems (T2SS) to secrete to their surface folded proteins that confer diverse functions, from nutrient acquisition to virulence. In the Klebsiella species, T2SS-mediated secretion of pullulanase (PulA) requires assembly of a dynamic filament called the endopilus. The inner membrane assembly platform (AP) subcomplex is essential for endopilus assembly and PulA secretion. AP components PulL and PulM interact with each other through their C-terminal globular domains and transmembrane segments. Here, we investigated the roles of their periplasmic helices, predicted to form a coiled coil, in assembly and function of the PulL-PulM complex. PulL and PulM variants lacking these periplasmic helices were defective for interaction in the bacterial two-hybrid (BACTH) assay. Their functions in PulA secretion and assembly of PulG subunits into endopilus filaments were strongly reduced. Interestingly, deleting the cytoplasmic peptide of PulM nearly abolished the function of variant PulMΔN and its interaction with PulG, but not with PulL, in the BACTH assay. Nevertheless, PulL was specifically proteolyzed in the presence of the PulMΔN variant, suggesting that PulM N-terminal peptide stabilizes PulL in the cytoplasm. We discuss the implications of these results for the T2S endopilus and type IV pilus assembly mechanisms.
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Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Experiments in molecular genetics
Miller, J. H. (1972) · Medical Entomology and Zoology · Cited by 27,157
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
Datsenko, K. A., & Wanner, B. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.120163297 · Cited by 14,782
Schägger, H., & von Jagow, G. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90587-2 · Cited by 11,444
Varadi, M., Anyango, S., Deshpande, M., Nair, S., Natassia, C., Yordanova, G., Yuan, D., Stroe, O., Wood, G., Laydon, A., et al. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkab1061 · Cited by 7,850
Cold Spring Harbor Laboratory; Cold Spring Harbor, NY
Cavalier-Smith, T. (1980) · FEBS Letters · doi:10.1016/0014-5793(80)80323-1 · Cited by 6,257
High efficiency transformation of Escherichia coli with plasmids
Inoue, H., Nojima, H., & Okayama, H. (1990) · Gene · doi:10.1016/0378-1119(90)90336-p · Cited by 2,304
A bacterial two-hybrid system based on a reconstituted signal transduction pathway
Karimova, G., Pidoux, J., Ullmann, A., & Ladant, D. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.10.5752 · Cited by 1,707
Pilus formation and protein secretion by the same machinery in Escherichia coli
Sauvonnet, N., Vignon, G., Pugsley, A. P., & Gounon, P. (2000) · The EMBO Journal · doi:10.1093/emboj/19.10.2221 · Cited by 233
Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond
Cianciotto, N. P., & White, R. C. (2017) · Infection and Immunity · doi:10.1128/iai.00014-17 · Cited by 226
Metadata
- Authors
- Li, Y., Santos-Moreno, J., & Francetic, O.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Venue
- Research in Microbiology
- Publisher
- Elsevier BV
- Published
- 2023-09
- Volume
- 174
- Issue
- 7
- Pages
- 104075
- ISSN
- 0769-2609 (ISSN-L)
- 0923-2508
- OpenAlex
- W4367671701
- Cited by
- 5
- References
- 55
- Citations by year
- 2026: 4 · 2025: 1
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- creativecommons.org/licenses/by-nc-nd/4.0/ (vor)
- Locations
- Research in Microbiology (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed (publishedVersion)
- Repositori digital de la UPF (Universitat Pompeu Fabra) (submittedVersion, cc-by-nc-nd)
- HAL (submittedVersion, cc-by-nc-nd)
- Funders
- Agence Nationale de la Recherche (ANR‐19, ANR-19-CE11-0020)
- Eusko Jaurlaritza
- China Scholarship Council
- Centre National de la Recherche Scientifique
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- type II secretion system, Klebsiella, pull, protein-protein interaction, proteolysis
- MeSH terms
- Type II Secretion Systems, Bacterial Proteins, Klebsiella, Membrane Proteins, Peptides
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- CoChat. (2026). Citation check: The periplasmic coiled coil formed by the assembly platform proteins PulL and PulM is critical for function of the Klebsiella type II secretion system. https://app.cochat.ai/papers/10.1016/j.resmic.2023.104075
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- Li, Y., Santos-Moreno, J., & Francetic, O. (2023). The periplasmic coiled coil formed by the assembly platform proteins PulL and PulM is critical for function of the Klebsiella type II secretion system. Research in Microbiology. https://doi.org/10.1016/j.resmic.2023.104075
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