Identification of groups of metabolically-active rhizosphere microorganisms by stable isotope probing of PLFAs
Abstract
We combined microbial community phospholipid fatty acid (PLFA) analyses with an in situ stable isotope 13CO2 labelling approach to identify microbial groups actively involved in assimilation of root-derived C in limed grassland soils. We hypothesized that the application of lime would stimulate more rapid 13C assimilation and turnover in microbial PLFAs. Four and 8 d after label application, 18:1omega9, 18:2omega6,9 (fungal biomarkers) and 16:1omega7, 18:1omega7, 19:0cy (Gram-negative bacterial biomarkers) showed the most 13C enrichment and rapid turnover rates. This suggests that these microorganisms were assimilating recently-photosynthesized root C inputs to soils. Contrary to our hypothesis, liming did not affect assimilation or turnover rates of 13C-labelled C. 13C stable isotope pulse-labelling technique paired with analyses of PLFA microbial biomarkers shows promise for in situ investigations of microbial function in soils.
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Particular specific monounsaturated fatty acids such as PLFA 16:1ω5, 16:1ω7, 17:1ω8 and 18:1ω7 are highly enriched by root-derived organic carbon in label experiments.
“Particular specific monounsaturated fatty acids such as PLFA 16:1 o 5, 16:1 o 7, 17:1 o 8 and 18:1 o 7 were shown to be highly enriched by root-derived organic carbon in label experiments 54,55 .”
From Plant diversity increases soil microbial activity and soil carbon storage (2015) · cites this paper as [55]
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References 20 · Cited by 249
- Authors
- Treonis, A. M., Ostle, N. J., Stott, A. W., Primrose, R., Grayston, S. J., & Ineson, P.
- Affiliations
- UK Centre for Ecology & Hydrology (GB)
- University of York (GB)
- Corresponding author
- Treonis, Amy M.
- Venue
- Soil Biology and Biochemistry
- Publisher
- Elsevier BV
- Published
- 2004-03
- Volume
- 36
- Issue
- 3
- Pages
- 533-537
- ISSN
- 0038-0717 (ISSN-L)
- OpenAlex
- W1998220314
- Cited by
- 249
- References
- 20
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 13 · 2023: 9 · 2022: 4 · 2021: 9 · 2020: 7 · 2019: 11 · 2018: 11 · 2017: 13 · 2016: 9 · 2015: 20 · 2014: 12 · 2013: 14 · 2012: 15
- FWCI
- 17.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Soil Biology and Biochemistry (publishedVersion)
- Funders
- Natural Environment Research Council
- Centre for Ecology and Hydrology
- Topics
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Carbon and Nitrogen Dynamics
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycorrhizal Fungi and Plant Interactions
- Keywords
- rhizosphere microorganisms, phospholipid fatty acids, stable isotope probing, 13C pulse labeling, root-derived carbon, carbon assimilation, carbon turnover, liming, grassland soils, fungal biomarkers, Gram-negative bacteria, microbial community function
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