Low Incidence of Off-Target Mutations in Individual CRISPR-Cas9 and TALEN Targeted Human Stem Cell Clones Detected by Whole-Genome Sequencing

Veres, Adrian, Gosis, Bridget S., Ding, Qiurong, Collins, Ryan, Ragavendran, Ashok, Brand, Harrison, Erdin, Serkan, Cowan, Chad A. +2 more·2014·Cell Stem Cell
DOI 10.1016/j.stem.2014.04.020

Cited by (474)

Items 401–474 of 474

  1. VCFshiny: an R/Shiny application for interactively analyzing and visualizing genetic variants
    Tao Chen, Chengcheng Tang, Wei Zheng, Yanan Qian, Min Chen, Qingjian Zou, Yinge Jin, Kepin Wang, Xiaoqing Zhou, Shixue Gou et al. · 2023 · Bioinformatics Advances
  2. Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
    Zhong‐Dong Shi, Chew-Li Soh, Zeng‐Rong Zhu, Danwei Huangfu · 2017 · Journal of Visualized Experiments
  3. The Challenge of a “Functional Cure” for AIDS by Gene Modified HSCT Therapy
    Lei Xu, Hu Chen, Bin Zhang · 2015 · Current Stem Cell Research & Therapy
  4. [Research advances on gene therapy for hemophilia A].
    Xiaolan Li, Jianping Zhang, Lei Zhang, Tao Cheng, Xiao‐Bing Zhang · 2015 · PubMed Central
  5. At the Conflux of Human Genome Engineering and Induced Pluripotency
    Knut Woltjen, Fabian Oceguera-Yañez, Harunobu Kagawa, Shin‐Il Kim · 2016 · Springer eBooks
  6. RISPR-Cas9: a weapon against COVID-19
    2021 · Biomedical Letters
  7. Gene Editing in Regenerative Medicine
    Yunlan Fang, Xuguang Chen, W.T. Godbey · 2018 · Elsevier eBooks
  8. Does Sharia Support Cloning? A Qualitative Analysis
    Sabbir Hasan · 2023 · International Journal of Islamic Khazanah
  9. CRISPR/Cas9 Editing in Induced Pluripotent Stem Cells: A Way Forward for Treating Cystic Fibrosis?
    Erik J. Quiroz, Amy L. Ryan · 2019 · Springer eBooks
  10. CRISPR Genome Editing Technology and its Application in Genetic Diseases: A Review
    Sepideh Khatibi, Amirhossein Sahebkar, Seyed Hamid Aghaee‐Bakhtiari · 2020 · Current Pharmaceutical Biotechnology
  11. Concise review: Inherited cardiac diseases, pluripotent stem cells, and genome editing combined-the past, present, and future
    Lettine van den Brink, Catarina Grandela, Christine L. Mummery, Richard P. Davis · 2019
  12. Assessing Off-Target Editing of CRISPR-Cas9 Systems
    Shondra M. Pruett‐Miller · 2020 · The CRISPR Journal
  13. Trio deep-sequencing does not reveal unexpected mutations in Cas9-edited monkeys
    Xin Luo, Yaoxi He, Chao Zhang, Xiechao He, Lanzhen Yan, Min Li, Ting Hu, Yan Hu, Jin Jiang, Xiaoyu Meng et al. · 2018 · bioRxiv (Cold Spring Harbor Laboratory)
  14. Analysis of splice-defect associated cardiac diseases using a patient-specific iPSC-cardiomyocyte system
    Sabine Rebs · 2021
  15. Progress in HIV-1 Prevention, Control and Treatment: Genetic Manipulation or Pharmacological Blockade of Chemokine Receptor 5?
    Abdullahi İbrahim Uba, Gonca Dilcan, Sani Sharif Usman · 2016 · Journal of Advances in Biology & Biotechnology
  16. Electroporation of CRISPR/Cas9 Targeting Neurogenin 3 ( NGN3 ) in Porcine Embryos and Its Effects on Mosaicism and Off-Target Effects by Next Generation Sequencing (NGS)
    Insung Park, Sergio Navarro‐Serna, Raquel M. Pinho, Trish Berger, Elizabeth A. Maga, Joaquín Gadea, Seung K. Kim, Pablo J. Ross · 2024 · Re GEN Open
  17. Comprehensive assessment of on- and off-target mutagenesis via lipid nanoparticle delivery of CRISPR-Cas9 genome editing
    Youichi Naoe, Naoko Fujimoto, Yukimasa Makita, Dongyang Li, Naoto Inukai, Akitsu Hotta · 2026 · Molecular Therapy — Nucleic Acids
  18. Genome Editing in Stem Cells
    Lorna M. FitzPatrick, Tristan R. McKay · 2015 · Current Stem Cell Reports
  19. Cell mediated gene therapy: A guide for doctors in the clinic
    Moon Jong Noh · 2015 · World Journal of Medical Genetics
  20. Genetic Engineering in Stem Cell Biomanufacturing
    Xiaoping Bao, Sean P. Palecek · 2016 · Elsevier eBooks
  21. Developments in the Generation of Reporter Stem Cells
    Samvel Avagyan, Kyle Sylakowski, Uma Lakshmipathy · 2016 · Springer eBooks
  22. Genome engineering in human pluripotent stem cells
    Udit Parekh, Marianna Yusupova, Prashant Mali · 2017 · Current Opinion in Chemical Engineering
  23. CRISPR/Cas9 Gene Editing in Mammalian Cells Using LentiCRISPRv2/LentiGuide-Puro Vectors
    Zubaida Said Ameen, Ece Çakıroğlu, Şerif Şentürk, Abdullahi Umar Ibrahim, Mehmet Özsöz · 2021 · Springer protocols handbooks/Springer protocols
  24. Genome Editing in Human Pluripotent Stem Cells
    Liuhong Cai, Yoon-Young Jang, Zhaohui Ye · 2016 · Stem cell biology and regenerative medicine
  25. CRISPR/Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells
    Jianping Zhang, Amanda Neises, Tao Cheng, Xiao‐Bing Zhang · 2015 · SpringerBriefs in stem cells
  26. Using CRISPR-Cas9 Genome Editing to Enhance Cell Based Therapies for the Treatment of Diabetes Mellitus
    Nicole A. J. Krentz, Francis C. Lynn · 2016 · Springer eBooks
  27. Forschung zu Genodermatosen durch neue Genom‐Editing‐Methoden
    Janin Lehmann, Christina Seebode, Steffen Emmert · 2017 · JDDG Journal der Deutschen Dermatologischen Gesellschaft
  28. Assessing for off-target mutagenesis
    Kiran Musunuru · 2021 · Elsevier eBooks
  29. Validation and Long-Term Follow Up of CD33 Off-Targets Predicted In Vitro and In Silico Using Error-Corrected Sequencing in Rhesus Macaques
    Aisha A. Aljanahi, Cícera Regina Lazzarotto, Shirley Chen, Tae–Hoon Shin, Stefan Cordes, Isabel Jabara, Yifan Zhou, David J. Young, Byung‐Chul Lee, Kyung–Rok Yu et al. · 2020 · bioRxiv (Cold Spring Harbor Laboratory)
  30. A CRISPR/Cas9 Based Strategy to Manipulate the Alzheimerrs Amyloid Pathway
    Jichao Sun, Jared Carlson-Stevermer, Utpal Das, Minjie Shen, Marion Delenclos, Amanda M. Snead, Lina Wang, Jonathan Loi, Andrew J. Petersen, Michael E. Stockton et al. · 2018 · SSRN Electronic Journal
  31. Specificity of CRISPR‐Cas9 Gene Editing
    Niklas Selfjord, Alexandra Madsen, Pınar Akçakaya · 2022 · Wiley eBooks
  32. Minimized combinatorial CRISPR screens identify genetic interactions in autophagy
    Valentina Diehl · 2022
  33. Construction and application of CRISPR-mediated TBCK-Knockout system in multiple human and mouse cell models
    Jin Wu · 2023 · Preprints.org
  34. Construction and application of CRISPR-mediated TBCK-Knockout system in multiple human and mouse cell models
    Jin Wu, JIANJUN ZHU, Andrew Dynka, JINGJIA CHANG, Xintong Zhang, JUN JIANG, Guanting Lu · 2023 · Preprints.org
  35. Hitting the mark
    Michael S. Eisenstein · 2014 · Nature Methods
  36. Developing Human Stem Cell Derived Motor Neuron Models of Amyotrophic Lateral Sclerosis
    Jackson Sandoe · 2014 · Digital Access to Scholarship at Harvard (DASH) (Harvard University)
  37. Scientific Review on Novel Genome Editing Techniques
    Kazunari Kondo, Kôsuke Nakamura · 2014 · Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
  38. Functional investigation of neural circuits in larval zebrafish
    Andrew W. Thompson · 2016 · The University of Queensland
  39. Patient-Derived Induced Pluripotent Stem Cells Provide a Regenerative Medicine Platform for Duchenne Muscular Dystrophy Heart Failure
    Xuan Guan, David L. Mack, Martin K. Childers · 2015 · Stem cell biology and regenerative medicine
  40. The Past, Present and Future of Gene Correction Therapy
    Duygu Akçay, Çetin Kocaefe · 2014 · Acta Medica
  41. Leveraging CRISPR/Cas Genome Editing Technology to Identify and Characterize Causal GWAS Variants for Blood Lipids
    Avanthi Raghavan · 2017 · Digital Access to Scholarship at Harvard (DASH) (Harvard University)
  42. Modeling Disease In Vivo With
    Eleanor Dow · 2015
  43. Using a novel cellular platform to optimize CRISPR/CAS9 technology for the gene therapy of AIDS
    Jingjin He, Thanutra Zhang, Xuemei Fu · 2017 · Protein & Cell
  44. Generation of knockout human iPSCs to investigate genes associated with telomere length
    Noor Shamkhi · 2017 · Figshare
  45. Experimental DNA- or RNA-Directed Therapies for Trinucleotide Repeat Disease
    Inês Lopes Cardoso · 2018 · OBM Genetics
  46. Precise genome editing human stem cells for functional interrogation of genetic variation
    Cory J. Smith · 2015
  47. Genome Editing Technologies: From Bench Side to Bedside
    Fulya Yaylacıoğlu Tuncay, Pervin Dinçer · 2018 · Acta Medica
  48. Clustered regularly interspaced short palindromic repeats-Cas9 gene therapy: The top 50 most-cited papers
    Jude Howaidi, Ali Howaidi, Faisal Howaidi · 2019 · Journal of Nature and Science of Medicine
  49. CRISPR-Mediated Knockout of EGFP: Part I
    Susan D. Biegel Carson, Heather B. Miller, D. Scott Witherow, Melissa C. Srougi · 2019 · Elsevier eBooks
  50. CRISPR-Cas9 Targeting of E7 Oncogene in HPV-positive Head and Neck Squamous Cell Carcinomas
    Vuk Bortnik · 2019 · Griffith Research Online
  51. Cut, Repair, Stitch – How Close Are We to Molecular Surgery Performed Using Genome Editing Techniques?
    Yichi Zhang, Aaron MacCosham · 2017 · Health Science Inquiry
  52. Verification of genetic engineering in yeasts with nanopore whole genome sequencing
    Joseph H. Collins, Kevin W. Keating, Trent R. Jones, Shravani Balaji, Celeste B. Marsan, Marina Çomo, Zachary J. Newlon, Tom Mitchell, Bryan Bartley, Aaron Adler et al. · 2020 · bioRxiv (Cold Spring Harbor Laboratory)
  53. Genome editing for cellular disease modeling
    Kiran Musunuru · 2021 · Elsevier eBooks
  54. Modelling adverse drug reactions using induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs)
    Effimia Christidi · 2021 · cIRcle (University of British Columbia)
  55. Gene Editing Through CRISPR-Based Technology
    Ziheng Zhang, Ping Wang, Ji‐Long Liu · 2022 · Springer eBooks
  56. Faculty Opinions recommendation of A mouse geneticist's practical guide to CRISPR applications.
    Søren Warming · 2018 · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
  57. Instances of Human Genetic Resistance Against Pathogens
    Joshua Neil Sampson, Pavithran T. Ravindran · 2021 · Journal of Student Research
  58. Molecular Mechanisms of Type II CRISPR ‐ Cas Systems
    Tautvydas Karvelis, Virginijus Šikšnys · 2022 · Wiley eBooks
  59. Gene editing hPSCs for modeling neurological disorders
    Andrew J. Petersen · 2022 · Elsevier eBooks
  60. Advancements in the Treatment of Transthyretin Amyloidosis
    Rena Wang, Chanyoung Park · 2023 · Journal of Student Research
  61. Novel CRISPR-Cas9 approach to combat with COVID-19
    Jahanzaib Ali · 2023 · BIOEDUSCIENCE
  62. WITHDRAWN: Codon-Optimized Base Editors Enable Efficient Base Substitution in Non-model Animals
    Jinhai Wang, Jiaqi Wang, Alexandra Florea, Robert Stewart, Maeve Ballantyne, Carl S. Tucker, Ross D. Houston, Diego Robledo · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
  63. Validation of caprine H11 and the Rosa26 platform for transgene integration via CRISPR-based system: investigations on stable transgene expression and genetic biosafety
    Yaoguang Zhang, Fei Hao, Yuan Gao, Weiguo Song, Chang Su, Xudong Guo, Dongjun Liu · 2025 · Functional & Integrative Genomics
  64. RESTRICT-seq enables time-gated CRISPR screens and uncovers novel epigenetic dependencies of SCC resistance
    Dreyton Amador, James M. Powers, Ashley G. Njiru, Zahra Ansari, Yvon Woappi · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
  65. Genome Editing and Human-Induced Pluripotent Stem Cells Facilitate Parkinson’s Disease Modeling
    Catalina M. Polanco, José Inzunza, Ivan Nalvarte, Jonathan Arias · 2025 · Stem cell biology and regenerative medicine
  66. CRISPR-Mediated Knockout of EGFP: Part I
    Susan D. Biegel Carson, Heather B. Miller, D. Scott Witherow, Melissa C. Srougi · 2025 · Elsevier eBooks
  67. Codon-optimized base editors enable efficient base substitution in Atlantic salmon
    Jinhai Wang, Jiaqi Wang, Alexandra Florea, Robert Stewart, Ross D. Houston, Diego Robledo · 2026 · Trends in biotechnology
  68. Preuve de concept de thérapie génique d’une dystrophie rétinienne en l’absence de modèle animal de la pathologie : cas de la Choroïdérémie
    Nicolas Cereso · 2014
  69. 3-D Genome organization of DNA damage repair
    Ujjwal Kumar Banerjee · 2017 · theses.fr (ABES)
  70. Etudes des translocations chromosomiques en utilisant les méthodes d'édition du génome : des mécanismes moléculaires à l’oncogenèse
    Loélia Babin · 2019
  71. Arrayed dual-gRNA CRISPR screening platform for C9orf72 repeat expansion excision in patient iPSCs
    Olubankole Aladesuyi Arogundade, Katie Jing Kay Lam, Katherine A. Brown, Tanya Jain, Patrick Oliver Issagholian-Lewin, Cerianne Huang, Taylor R. Hudson, Kevin Briseno, Stacia K. Wyman, Netravathi Krishnappa et al. · 2026 · Molecular Therapy Advances
  72. Multiomic Profiling of 85 iPSC Lines from Familial Dementia Reveals Cellular Diversity and Regulators of Organoid Development
    Le Qi, Taylor B. Bertucci, Susan M. Borden, Brigitte L. Arduini, Steven Lotz, Kathryn R. Bowles, Alison Goate, Celeste M M. Karch, Sally Temple, Daniel H. Geschwind · 2026 · SSRN Electronic Journal
  73. Gene-Edited Stem Cells for Ischemic Vascular Disease: Current Advances and Future Perspectives
    Seongho Han, Sung-Whan Kim · 2026 · Current Issues in Molecular Biology
  74. Multi-omic profiling of 85 iPSC lines from familial dementia reveals cellular diversity and regulators of organoid development
    Le Qi, Taylor B. Bertucci, Susan M. Borden, Brigitte L. Arduini, Steven Lotz, Kathryn R. Bowles, Alison Goate, Celeste M M. Karch, Sally Temple, Daniel H. Geschwind · 2026 · Neuron