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Higher-Density Culture in Human Embryonic Stem Cells Results in DNA Damage and Genome Instability

Jacobs, Kurt, Zambelli, Filippo, Mertzanidou, Afroditi, Smolders, Ilse, Geens, Mieke, Nguyen, Ha Thi, Barbé, Lise, Sermon, Karen +1 more·2016·Stem Cell Reports
DOI 10.1016/j.stemcr.2016.01.015PMC PMC4788786
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  • Such genetic changes appear to occur at any time during passage and may be associated with culture conditions such as medium acidification, frequent single cell dissociation, or repeated use of Rho kinase inhibitors.

    “Such changes appear to occur at any time during passage (International Stem Cell Initiative, 2011) and may be associated with culture conditions - such as medium acidification - as a consequence of suboptimal culturing and passaging (e.g., frequent single cell dissociation) or the repeated use of Rho kinase inhibitors (Assou et al., 2018; Jacobs et al., 2016).”

    From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Jacobs et al., 2016

    The Results section reports that medium acidification caused by lactate accumulation was a key factor in DNA damage in high-density hESC cultures; the Discussion links suboptimal culture conditions to genomic instability. The paper does not establish frequent single-cell dissociation or repeated Rho kinase inhibitor use as causes. The claim about changes occurring at any time during passage is cited to a different source, not Jacobs et al. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Jacobs, K., Zambelli, F., Mertzanidou, A., Smolders, I., Geens, M., Nguyen, H. T., Barbé, L., Sermon, K., & Spits, C.
Venue
Stem Cell Reports
Publisher
Elsevier BV
Published
2016-03
Volume
6
Issue
3
Pages
330-341
ISSN
2213-6711
DOI
10.1016/j.stemcr.2016.01.015
PMC
PMC4788786
Cited by
80
References
2
Language
en
Licence
Funders
  • Scientific Research Flanders
  • Methusalem
  • Vrije Universiteit Brussel
  • Leerstoel Mireille Aerens

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