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Cited by 77Pancreatic function and diabetesPluripotent Stem Cells ResearchDiabetes and associated disorders

Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells

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Trott, Jamie, Tan, Ee Kim, Ong, Sheena, Titmarsh, Drew M., Denil, Simon L.I.J., Giam, Maybelline, Wong, Cheng Kit, Wang, Jiaxu +8 more·2017·Stem Cell Reports
DOI 10.1016/j.stemcr.2017.05.019PMC PMC5470345
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Abstract

Pluripotent stem cells have been proposed as an unlimited source of pancreatic β cells for studying and treating diabetes. However, the long, multi-step differentiation protocols used to generate functional β cells inevitably exhibit considerable variability, particularly when applied to pluripotent cells from diverse genetic backgrounds. We have developed culture conditions that support long-term self-renewal of human multipotent pancreatic progenitors, which are developmentally more proximal to the specialized cells of the adult pancreas. These cultured pancreatic progenitor (cPP) cells express key pancreatic transcription factors, including PDX1 and SOX9, and exhibit transcriptomes closely related to their in vivo counterparts. Upon exposure to differentiation cues, cPP cells give rise to pancreatic endocrine, acinar, and ductal lineages, indicating multilineage potency. Furthermore, cPP cells generate insulin+ β-like cells in vitro and in vivo, suggesting that they offer a convenient alternative to pluripotent cells as a source of adult cell types for modeling pancreatic development and diabetes.

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  • Several studies have demonstrated the ability to propagate the pancreatic progenitor population generated during the middle portion of the differentiation protocol.

    “Several studies, however, have demonstrated the ability to propagate the pancreatic progenitor population that is generated during the middle portion of the differentiation protocol.181,182”

    From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [181]

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Authors
Trott, J., Tan, E. K., Ong, S., Titmarsh, D. M., Denil, S. L. I. J., Giam, M., Wong, C. K., Wang, J., Shboul, M., Eio, M., Cooper-White, J., Cool, S. M., Rancati, G., Stanton, L. W., Reversade, B., & Dunn, N. R.
Affiliations
  • Agency for Science, Technology and Research (SG)
  • The University of Queensland (AU)
Corresponding author
  • Trott, Jamie
  • Dunn, N. Ray
ORCID
Venue
Stem Cell Reports
Publisher
Elsevier BV
Published
2017-06
Volume
8
Issue
6
Pages
1675-1688
ISSN
2213-6711 (ISSN-L)
DOI
10.1016/j.stemcr.2017.05.019
PMC
PMC5470345
OpenAlex
W2621496209
Cited by
77
References
45
Citations by year
2026: 4 · 2025: 4 · 2024: 15 · 2023: 10 · 2022: 9 · 2021: 11 · 2020: 9 · 2019: 8 · 2018: 5 · 2017: 2
FWCI
6.73
Type
journal article
Language
en
Open access
Licence
Locations
  • Stem Cell Reports (publishedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • DR-NTU (Nanyang Technological University) (submittedVersion, cc-by-nc-nd)
Funders
  • Agency for Science, Technology and Research
  • Economic Development Board - Singapore
Topics
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
Keywords
pancreatic progenitor cells, human pluripotent stem cells, self-renewal, long-term culture, beta cell differentiation, insulin-producing cells, PDX1, SOX9, pancreatic development, diabetes modeling, multilineage differentiation, pancreatic endocrine cells
MeSH terms
Cell Self Renewal, Animals, Cell Differentiation, Cell Line, Humans, Insulin, Kidney, Pancreas, Stem Cells, Transplantation, Heterologous, Trans-Activators, Down-Regulation, Mice, SCID, Mice, Inbred NOD, Homeodomain Proteins, Pluripotent Stem Cells, Insulin-Secreting Cells, Mice, SOX9 Transcription Factor, Feeder Cells

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