The DOI matches its Crossref record.
Movies of Ice-Embedded Particles Enhance Resolution in Electron Cryo-Microscopy
Open accessAbstract
Summary Low-dose images obtained by electron cryo-microscopy (cryo-EM) are often affected by blurring caused by sample motion during electron beam exposure, degrading signal especially at high resolution. We show here that we can align frames of movies, recorded with a direct electron detector during beam exposure of rotavirus double-layered particles, thereby greatly reducing image blurring caused by beam-induced motion and sample stage instabilities. This procedure increases the efficiency of cryo-EM imaging and enhances the resolution obtained in three-dimensional reconstructions of the particle. Employing movies in this way is generally applicable to all cryo-EM samples and should also improve the performance of mid-range electron microscopes that may have limited mechanical stability and beam coherence.
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Cited by
Structural insights into coronavirus entry
(2019) · Advances in Virus Research · doi:10.1016/bs.aivir.2019.08.002 · Cited by 769
Metadata
- Authors
- Campbell, M. G., Cheng, A., Brilot, A. F., Moeller, A., Lyumkis, D., Veesler, D., Pan, J., Harrison, S. C., Potter, C. S., Carragher, B., & Grigorieff, N.
- Venue
- Structure
- Publisher
- Elsevier BV
- Published
- 2012-11
- Volume
- 20
- Issue
- 11
- Pages
- 1823-1828
- ISSN
- 0969-2126 (ISSN-L)
- OpenAlex
- W1987905625
- Cited by
- 310
- References
- 0
- FWCI
- 13.04
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
