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Cited by 3Lipid Membrane Structure and BehaviorProtein Structure and DynamicsMicrotubule and mitosis dynamics

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Anillin-related Mid1 as an adaptive and multimodal contractile ring anchoring protein: A simulation study

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Hall, Aaron R., Choi, Yeol Kyo, Im, Wonpil, Vavylonis, Dimitrios·2024·Structure
DOI 10.1016/j.str.2023.11.010PMC PMC10872332

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Abstract

Cytokinesis of animal and fungi cells depends crucially on the anillin scaffold proteins. Fission yeast anillin-related Mid1 anchors cytokinetic ring precursor nodes to the membrane. However, it is unclear if both of its Pleckstrin Homology (PH) and C2 C-terminal domains bind to the membrane as monomers or dimers, and if one domain plays a dominant role. We studied Mid1 membrane binding with all-atom molecular dynamics near a membrane with yeast-like lipid composition. In simulations with the full C terminal region started away from the membrane, Mid1 binds through the disordered L3 loop of C2 in a vertical orientation, with the PH away from the membrane. However, a configuration with both C2 and PH initially bound to the membrane remains associated with the membrane. Simulations of C2-PH dimers show extensive asymmetric membrane contacts. These multiple modes of binding may reflect Mid1's multiple interactions with membranes, node proteins, and ability to sustain mechanical forces.

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Metadata

Authors
Hall, A. R., Choi, Y. K., Im, W., & Vavylonis, D.
Affiliations
Lehigh University (US)
Corresponding author
Vavylonis, Dimitrios
ORCID
Venue
Structure
Publisher
Elsevier BV
Published
2024-02
Volume
32
Issue
2
Pages
242-252.e2
ISSN
0969-2126 (ISSN-L)
DOI
10.1016/j.str.2023.11.010
PMC
PMC10872332
OpenAlex
W4389781434
Cited by
3
References
3
Citations by year
2026: 1 · 2025: 1 · 2024: 1
FWCI
0.27
Type
journal article
Language
en
Open access
Licence
Locations
  • Structure (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • PubMed Central (submittedVersion)
Funders
  • National Institutes of Health (R35GM136372)
  • Office of Science (2019035, DE-AC02-06CH11357)
  • National Science Foundation (MCB-2111728, MCB180021)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
MID1, anillin, contractile ring, cytokinesis, Schizosaccharomyces pombe, pleckstrin homology domain, C2 domain, membrane binding, protein-membrane interactions, mechanical forces

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