Methods to study the tumor microenvironment under controlled oxygen conditions
Open accessAbstract
The tumor microenvironment (TME) is a complex heterogeneous assembly composed of a variety of cell types and physical features. One such feature, hypoxia, is associated with metabolic reprogramming, the epithelial-mesenchymal transition, and therapeutic resistance. Many questions remain regarding the effects of hypoxia on these outcomes; however, only a few experimental methods enable both precise control over oxygen concentration and real-time imaging of cell behavior. Recent efforts with microfluidic platforms offer a promising solution to these limitations. In this review, we discuss conventional methods and tools used to control oxygen concentration for cell studies, and then highlight recent advances in microfluidic-based approaches for controlling oxygen in engineered platforms.
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References 102 · Cited by 104
Research works printed but not linked: 1
- [75]Hoeckel M Vaupel P Tumor hypoxia: Definitions and current clinical, biologic, and molecular aspects Journal of the National Cancer Institute (Bethesda) 2001 93 266 276 11181773
- Authors
- Byrne, M. B., Leslie, M. T., Gaskins, H. R., & Kenis, P. J. A.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Corresponding author
- Gaskins, H. Rex
- Kenis, Paul J.A.
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2014-11
- Volume
- 32
- Issue
- 11
- Pages
- 556-563
- ISSN
- 0167-7799 (ISSN-L)
- PMC
- PMC4254115
- OpenAlex
- W2033037709
- Cited by
- 104
- References
- 102
- Citations by year
- 2026: 6 · 2025: 4 · 2024: 4 · 2023: 10 · 2022: 21 · 2021: 14 · 2020: 10 · 2019: 7 · 2018: 11 · 2017: 8 · 2016: 5 · 2015: 4
- FWCI
- 0.48
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Trends in biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Cancer Institute (R33CA137719)
- National Institute of General Medical Sciences (R01GM083601)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Keywords
- tumor microenvironment, hypoxia, oxygen concentration control, microfluidic platform, metabolic reprogramming, epithelial-mesenchymal transition, treatment resistance, real-time imaging
- MeSH terms
- Hypoxia, Cytological Techniques, Humans, Neoplasms, Oxygen, Microfluidics, Tumor Microenvironment
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