Estrogen receptor expression affected by hypoxia inducible factor-1α in stromal cells from patients with endometriosis
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OBJECTIVE: Endometriosis is an estrogen-dependent disease. The aim of this study was to evaluate the different expression of estrogen receptors (ER) and its relation to hypoxia inducible factor-1α (HIF-1α) in stromal cells from women with endometriosis. MATERIALS AND METHODS: Paired eutopic endometrial and ectopic endometriotic stromal cells were isolated from women with endometriosis while they underwent laparoscopy. The expression of ERα and ERβ was measured by reverse transcription-polymerase chain reaction and Western blot. Regulation of ER expression was evaluated by HIF-1α knockdown via short interference RNA. RESULTS: The expression of ERβ was significantly increased in ectopic stromal cells. Treatment of endometrial stromal cells with hypoxia induced ERβ expression. Knockdown of HIF-1α abolished hypoxia-induced ERβ expression and increased ERα expression. CONCLUSION: The expression of ERβ is regulated by hypoxia. Results of this study will provide important information in the involvement of hypoxia factors in mediating estrogen action via different ER expression in endometriosis.
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Activation of HIF-1α and thus hypoxia can lead to increased estrogen production through depressing DUSP2 expression and thus COX-2 overexpression in endometriotic stromal cells, as well as increased ERβ expression.
“Our results are consistent with the finding that activation of HIF-1α and thus hypoxia can lead to increased estrogen production through depressing DUSP2 expression and thus COX-2 overexpression in endometriotic stromal cells49, as well as increased ERβ expression66.”
From Platelets induce increased estrogen production through NF-κB and TGF-β1 signaling pathways in endometriotic stromal cells (2020) · cites this paper as [66]
Increased HIF-1α accumulation results in DUSP2 down-regulation, which results in prolonged activation of ERK and p38 MAPK and increased COX-2 expression in endometriotic stromal cells, as well as increased ERβ expression which can also be induced by activated platelets.
“Increased HIF-1α accumulation results in DUSP2 down-regulation, which results in prolonged activation of ERK and p38 MAPK and increased COX-2 expression in endometriotic stromal cells49, as well as increased ERβ expression66 which can also be induced by activated platelets.”
From Platelets induce increased estrogen production through NF-κB and TGF-β1 signaling pathways in endometriotic stromal cells (2020) · cites this paper as [66]
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References 15 · Cited by 33
Research works printed but not linked: 2
- Wu MH, Lu CW, Chuang PC, Tsai SJ. Prostaglandin E2: the master of endometriosis? Exp Biol Med (Maywood) 2010;235:668e77.
- Wu MH, Huang MF, Chang FM, Tsai SJ. Leptin on peritoneal macro- phages of patients with endometriosis. Am J Reprod Immunol 2010;63: 214e21.
- Authors
- Wu, M.-H., Lu, C.-W., Chang, F.-M., & Tsai, S.-J.
- Affiliations
- National Cheng Kung University (TW)
- Corresponding author
- Tsai, Shaw-Jenq
- Venue
- Taiwanese Journal of Obstetrics and Gynecology
- Publisher
- Elsevier BV
- Published
- 2012-03
- Volume
- 51
- Issue
- 1
- Pages
- 50-54
- ISSN
- 1028-4559 (ISSN-L)
- OpenAlex
- W2063694007
- Cited by
- 33
- References
- 15
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 6 · 2019: 3 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 6 · 2014: 2 · 2013: 1
- FWCI
- 3.35
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Taiwanese Journal of Obstetrics and Gynecology (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Natural Science Council of Republic (NSC100-2314-B-006-012)
- National Cheng-Kung University Hospital (NCKUH-99-04007)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Obstetrics and Gynecology › Gynecological conditions and treatments
- Keywords
- endometriosis, estrogen receptor alpha, estrogen receptor beta, hypoxia-inducible factor-1α, endometrial stromal cells, ectopic endometrium, hypoxia, estrogen signaling, gene expression regulation
- MeSH terms
- Endometriosis, Endometrium, Female, Humans, RNA, Messenger, Cell Hypoxia, Stromal Cells, RNA, Small Interfering, Estrogen Receptor alpha, Estrogen Receptor beta, Hypoxia-Inducible Factor 1, alpha Subunit
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