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Cited by 7Clostridium difficile and Clostridium perfringens researchEscherichia coli research studiesMicrobial infections and disease research

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Non-toxic perfringolysin O and α-toxin derivatives as potential vaccine candidates against bovine necrohaemorrhagic enteritis

Verherstraeten, S., Goossens, E., Valgaeren, B., Pardon, B., Timbermont, L., Haesebrouck, F., Ducatelle, R., Deprez, P., Van Immerseel, F.·2016·The Veterinary Journal
DOI 10.1016/j.tvjl.2016.09.008
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Abstract

Bovine necrohaemorrhagic enteritis is a fatal Clostridium perfringens type A-induced disease that is characterised by sudden death. Recently the involvement of perfringolysin O and α-toxin in the development of necrohaemorrhagic lesions in the gut of calves was suggested, and thus derivatives of these toxins are potentially suitable as vaccine antigens. In the current study, the perfringolysin O derivative PFO L491D , alone or in combination with α-toxin derivative GST-cpa 247-370 , was evaluated as possible vaccine candidate, using in vitro assays. PFO L491D showed no haemolytic effect on horse red blood cells and no cytotoxic effect on bovine endothelial cells. Furthermore, calves immunised with PFO L491D raised antibodies against perfringolysin O that could inhibit the perfringolysin O-associated haemolytic activity on horse red blood cells. Antisera from calves immunised with PFO L491D had a significantly higher neutralising capacity against the cytotoxic effect of C. perfringens culture supernatant to bovine endothelial cells than serum from control calves (P L491D in combination with GST-cpa 247-370 elicited antibodies against perfringolysin O and α-toxin and consequently inhibited both the perfringolysin O-associated haemolytic activity and the α-toxin-associated lecithinase activity in vitro. Additionally, the neutralising ability of these antisera on the cytotoxic effect of C. perfringens culture supernatant to bovine endothelial cells was significantly higher than t…

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Research works printed but not linked: 45

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Metadata

Authors
Verherstraeten, S., Goossens, E., Valgaeren, B., Pardon, B., Timbermont, L., Haesebrouck, F., Ducatelle, R., Deprez, P., & Van Immerseel, F.
Affiliations
Ghent University (BE)
ORCID
Venue
The Veterinary Journal
Publisher
Elsevier BV
Published
2016-11
Volume
217
Pages
89-94
ISSN
1090-0233 (ISSN-L)
DOI
10.1016/j.tvjl.2016.09.008
OpenAlex
W2527743754
Cited by
7
References
0
Citations by year
2026: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2017: 1
FWCI
0.31
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • The Veterinary Journal (publishedVersion)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion)
Funders
Agency for Innovation by Science and Technology (090910)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
Clostridium perfringens type A, perfringolysin O, alpha-toxin, vaccine candidate, GST fusion protein, neutralizing antibodies, hemolytic activity, lecithinase activity, cytotoxicity, calves
MeSH terms
Animals, Bacterial Toxins, Bacterial Vaccines, Calcium-Binding Proteins, Cattle, Cattle Diseases, Clostridium Infections, Clostridium perfringens, Enteritis, Erythrocytes, Hemolysin Proteins, Immunity, Active, Type C Phospholipases, Vaccines, Synthetic, Endothelial Cells, Antibodies, Neutralizing

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