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Cited by 35Brucella: diagnosis, epidemiology, treatmentMicrobial infections and disease researchSalmonella and Campylobacter epidemiology

Brucellosis outbreak in a Swedish kennel in 2013: Determination of genetic markers for source tracing

Kaden, Rene, Ågren, Joakim, Båverud, Viveca, Hallgren, Gunilla, Ferrari, Sevinc, Börjesson, Joann, Lindberg, Martina, Bäckman, Stina +1 more·2014·Veterinary Microbiology
DOI 10.1016/j.vetmic.2014.10.015
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Abstract

Brucellosis is a highly infectious zoonotic disease but rare in Sweden. Nonetheless, an outbreak of canine brucellosis caused by an infected dog imported to Sweden was verified in 2013. In total 25 dogs were tested at least duplicated by the following approaches: real-time PCR for the detection of Brucella canis, a Brucella genus-specific real-time PCR, selective cultivation, and microscopic examination. The whole genome of B. canis strain SVA13 was analysed regarding genetic markers for epidemiological examination. The genome of an intact prophage of Roseobacter was detected in B. canis strain SVA13 with whole genome sequence prophage analysis (WGS-PA). It was shown that the prophage gene content in the American, African and European isolates differs remarkably from the Asian strains. The prophage sequences in Brucella may therefore serve of use as genetic markers in epidemiological investigations. Phage DNA fragments were also detected in clustered, regularly interspaced short palindromic repeats (CRISPR) in the genome of strain SVA13. In addition to the recommendations for genetic markers in Brucella outbreak tracing, our paper reports a validated two-step stand-alone real-time PCR for the detection of B. canis and its first successful use in an outbreak investigation.

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  • In many Southern European countries, there is a risk of exposure to diseases not encountered in the northern, importing countries; animals could be infected with Anaplasma phagocytophilum, Babesia canis, Brucella canis, Borrelia burgdorferi, Dirofilaria immitis, Dirofilaria repens, Echinococcus multilocularis, Echinococcus granulosus, Ehrlichia canis, Hepatozoon canis, Leishmania infantum, Linguat

    “In many Southern European countries, there is also a risk of exposure to diseases not encountered in the northern, importing, countries. Animals could be infected with Anaplasma phagocytophilum, Babesia canis, Brucella canis, Borrelia burgdorferi, Dirofilaria immitis (heartworm), Dirofilaria repens (subcutaneous worm), Echinococcus multilocularis (fox tapeworm), Echinococcus granulosus (hydatid or”

    From A One Health Perspective on the Human-Companion Animal Relationship with Emphasis on Zoonotic Aspects. (2020) · cites this paper as [195]

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Authors
Kaden, R., Ågren, J., Båverud, V., Hallgren, G., Ferrari, S., Börjesson, J., Lindberg, M., Bäckman, S., & Wahab, T.
Affiliations
  • Swedish Veterinary Agency (SE)
  • National Food Administration (SE)
  • Swedish Defence Research Agency (SE)
  • Public Health Agency of Sweden (SE)
ORCID
Venue
Veterinary Microbiology
Publisher
Elsevier BV
Published
2014-12
Volume
174
Issue
3-4
Pages
523-530
ISSN
0378-1135 (ISSN-L)
DOI
10.1016/j.vetmic.2014.10.015
OpenAlex
W1998774789
Cited by
35
References
0
Citations by year
2026: 4 · 2025: 3 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 6 · 2017: 2 · 2016: 2
FWCI
1.96
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Veterinary Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Veterinary › Small Animals › Brucella: diagnosis, epidemiology, treatment
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
Keywords
canine brucellosis, Brucella canis, outbreak investigation, source tracking, genetic markers, prophages, CRISPR, real-time PCR, kennels, zoonoses
MeSH terms
Animals, Base Sequence, Brucellosis, Disease Outbreaks, Dog Diseases, Dogs, Female, Genetic Markers, Humans, Male, Molecular Sequence Data, Species Specificity, Sweden, Zoonoses, Genome, Bacterial, Sequence Analysis, DNA, Brucella canis, Real-Time Polymerase Chain Reaction

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