First identification of the F200Y SNP in the β-tubulin gene linked to benzimidazole resistance in Ancylostoma caninum
Abstract
Single nucleotide polymorphisms (SNPs) in the β-tubulin isotype 1 gene in codons 167, 198 and 200 have been associated with benzimidazole resistance in some nematodes, although no polymorphisms in this gene have been described in the hookworm Ancylostoma caninum to date. This study aimed to screen for SNPs in the β-tubulin isotype 1 gene at codons 198 and 200 associated with benzimidazole resistance in A. caninum worms recovered from naturally infected dogs from two Brazilian states (Minas Gerais and Piauí). To perform this analysis, a molecular technique based on the amplification refractory mutation system (ARMS-PCR) was standardized. A total of 110 individual worms recovered from 9 dogs in Piauí and 124 individual worms recovered from 10 dogs in Minas Gerais were analyzed using this methodology. The presence of a SNP at position 200 was observed at a low frequency (0.8%), which was only detected in the worm population from Minas Gerais State. A total of 75 randomly selected samples were sequenced to validate this technique, and the presence of the SNP was confirmed. Furthermore, SNP at position 198 were evaluated by sequencing, and none were detected in this region. This is the first report of the presence of a SNP in the β-tubulin isotype 1 gene associated with benzimidazole resistance in an A. caninum population.
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This single nucleotide polymorphism in the β-tubulin isotype 1 gene was also found in Ancylostoma caninum.
“This single nucleotide polymorphism in the β-tubulin isotype 1 gene was also found in Ancylostoma caninum [331].”
From Albendazole and Mebendazole as Anti-Parasitic and Anti-Cancer Agents: an Update (2021) · cites this paper as [331]
Earlier studies on drug resistance to benzimidazoles were performed on intestinal nematodes of veterinary importance, in particular, Haemonchus contortus; the drug resistance in this nematode was found to be correlated with a single nucleotide polymorphism at codons 167, 198, or 200 of the β-tubulin isotype 1 gene.
“Earlier studies on the drug resistance to benzimidazoles were performed on intestinal nematodes of veterinary importance, in particular, Haemonchus contortus; the drug resistance in this nematode was found to be correlated with a single nucleotide polymorphism at codons 167, 198, or 200 of the β-tubulin isotype 1 gene [331,332].”
From Albendazole and Mebendazole as Anti-Parasitic and Anti-Cancer Agents: an Update (2021) · cites this paper as [331]
Studies performed in Brazil reported finding a SNP at codon 198 in A. braziliense and at codon 200 in A. caninum at very low frequencies, 1.2% and 0.8%, respectively, using PCR-RFLP.
“However, studies performed in Brazil did report finding a SNP at codon 198 in A. braziliense [87] and at codon 200 in A. caninum [88] at very low frequencies, 1.2 and 0.8%, respectively, using PCR-RFLP.”
From Multiple drug resistance in the canine hookworm Ancylostoma caninum: an emerging threat? (2019) · cites this paper as [88]
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References 21 · Cited by 58
- Authors
- Furtado, L. F. V., Bello, A. C. P. D. P., dos Santos, H. A., Carvalho, M. R. S., & Rabelo, É. M. L.
- Affiliations
- Universidade Federal de Minas Gerais (BR)
- Corresponding author
- Rabelo, Élida Mara Leite
- Venue
- Veterinary Parasitology
- Publisher
- Elsevier BV
- Published
- 2014-12
- Volume
- 206
- Issue
- 3-4
- Pages
- 313-316
- ISSN
- 0304-4017 (ISSN-L)
- OpenAlex
- W2071040688
- Cited by
- 58
- References
- 21
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 9 · 2023: 7 · 2022: 7 · 2021: 3 · 2020: 5 · 2019: 6 · 2018: 4 · 2017: 2 · 2016: 3 · 2015: 1
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Veterinary Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- PRPq/UFMG
- CNPq
- FAPEMIG
- Topics
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Nematode management and characterization studies
- Keywords
- Ancylostoma caninum, hookworm, benzimidazole resistance, beta-tubulin gene, single nucleotide polymorphisms, anthelmintic resistance, dogs, allele-specific PCR, Minas Gerais, Piauí
- MeSH terms
- Ancylostoma, Ancylostomiasis, Animals, Anthelmintics, Benzimidazoles, Drug Resistance, Female, Male, Tubulin, Polymorphism, Single Nucleotide
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