Premature processing of mouse mammary tumor virus Gag polyprotein impairs intracellular capsid assembly
Open accessAbstract
Mouse mammary tumor virus (MMTV) is the prototypical member of the Betaretrovirus genus, but the processes of its morphogenesis are poorly characterized. In this report, we describe an unusual intracellular processing of MMTV Gag polyprotein in human 293T cells transiently expressing MMTV from heterologous promoter. The same specific cleavage products of the viral protease were seen for the wild type as well as for nonmyristylated mutant of MMTV Gag polyprotein completely defective in the particle release. Inactivation of the viral protease resulted in more stable Gag polyprotein and in accumulation of intracytoplasmic particles for nonmyristylated Gag. The intracellular processing of nonmyristylated MMTV Gag indicates that protease activation in betaretrovirus can occur independently of budding.
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Even in the absence of other viral components, especially PR, expressing MMTV, FIV, and SIV Gag polyproteins independently directs the virus to the appropriate assembly pathway.
“Even in the absence of other viral components, especially PR, expressing MMTV, FIV, and SIV Gag polyproteins independently directs the virus to the appropriate assembly pathway”
From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Zábranský et al. 2009
Cites
Göttlinger, H. G., Sodroski, J. G., & Haseltine, W. A. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.15.5781 · Cited by 841 · see citing works
Karacostas, V., Wolffe, E. J., Nagashima, K., Gonda, M. A., & Moss, B. (1993) · Virology · doi:10.1006/viro.1993.1174 · Cited by 212 · see citing works
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Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication
Jehad, M. A., Ali, L. M., Pillai, V. N., Prabhu, S. G., Mustafa, F., & Rizvi, T. A. (2026) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuaf066 · Cited by 5 · see citing works
“Even in the absence of other viral components, especially PR, expressing MMTV, FIV, and SIV Gag polyproteins independently directs the virus to the appropriate assembly pathway (Zábranský et al. 2009 , Krishnan et al. 2019 , Pillai et al. 2023 ).”
- Authors
- Zábranský, A., Hadravová, R., Štokrová, J., Sakalian, M., & Pichová, I.
- Affiliations
- Czech Academy of Sciences (CZ)
- University of Oklahoma Health Sciences Center (US)
- Corresponding author
- Zábranský, Aleš
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 2009-02
- Volume
- 384
- Issue
- 1
- Pages
- 33-37
- ISSN
- 0042-6822 (ISSN-L)
- OpenAlex
- W1976438660
- Cited by
- 18
- References
- 2
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2022: 1 · 2021: 1 · 2019: 2 · 2016: 3 · 2015: 1 · 2013: 1 · 2012: 2
- FWCI
- 0.42
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 1627 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Virology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Akademie Věd České Republiky (1M6138896301, Z4 0550506)
- American Cancer Society
- Ministerstvo Školství, Mládeže a Tělovýchovy (RSG-06-0658-01-MBC)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Keywords
- mouse mammary tumor virus, Gag protein, intracellular processing, capsid assembly, protease activation, myristoylation, betaretrovirus, viral morphogenesis, particle release, HEK293T cells
- MeSH terms
- Animals, Dexamethasone, Female, Humans, Kinetics, Mammary Glands, Animal, Mammary Tumor Virus, Mouse, Mammary Neoplasms, Experimental, Peptide Hydrolases, Promoter Regions, Genetic, Proviruses, T-Lymphocytes, Transfection, Restriction Mapping, Mammary Neoplasms, Animal, Gene Products, gag, Amino Acid Substitution, Mice
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