Molecular and functional analysis of apical junction formation in the gut epithelium of Caenorhabditis elegans
Open accessAbstract
The Caenorhabditis elegans intestine is a simple and accessible model system to analyze the mechanism of junction assembly. In comparison to Drosophila and vertebrates, the C. elegans apical junction is remarkable because a single electron-dense structure is implicated in complex processes such as epithelial tightness, vectorial transport and cell adhesion. Here we present evidence in support of a heterogeneous molecular assembly of junctional proteins found in Drosophila and vertebrate epithelia associated with different junctions or regions of the plasma membrane. In addition, we show that molecularly diverse complexes participate in different aspects of epithelial maturation in the C. elegans intestine. DLG-1 (Discs large) acts synergistically with the catenin-cadherin complex (HMP-1-HMP-2-HMR-1) and the Ezrin-Radixin-Moesin homolog (ERM-1) to ensure tissue integrity of the intestinal tube. The correct localization of DLG-1 itself depends on AJM-1, a coiled-coil protein. Double depletion of HMP-1 (alpha-catenin) and LET-413 (C. elegans homolog of Drosophila Scribble) suggests that the catenin-cadherin complex is epistatic to LET-413, while additional depletion of subapically expressed CRB-1 (Crumbs) emphasizes a role of CRB-1 concerning apical junction formation in the C. elegans intestine.
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Some effects of LET-413 knockdown are alleviated by reducing cadherin function.
“In fact, some effects of LET-413 knockdown are alleviated by reducing cadherin function (Segbert et al., 2004).”
From Scribble: A master scaffold in polarity, adhesion, synaptogenesis, and proliferation (2019) · cites this paper as Segbert et al., 2004
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References 53 · Cited by 72
- Authors
- Segbert, C., Johnson, K., Theres, C., van Fürden, D., & Bossinger, O.
- Affiliations
- Heinrich Heine University Düsseldorf (DE)
- Corresponding author
- Bossinger, Olaf
- Venue
- Developmental Biology
- Publisher
- Elsevier BV
- Published
- 2004-02
- Volume
- 266
- Issue
- 1
- Pages
- 17-26
- ISSN
- 0012-1606 (ISSN-L)
- OpenAlex
- W2130786065
- Cited by
- 72
- References
- 53
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 3 · 2019: 4 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 5 · 2014: 5 · 2013: 1 · 2012: 7
- FWCI
- 1.15
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Developmental Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Boehringer Ingelheim Fonds
- Deutsche Forschungsgemeinschaft (SFB 590)
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- apical junctions, Caenorhabditis elegans intestine, epithelial tight junctions, DLG1, Discs large, Scribble, Crumbs, coiled-coil proteins, epithelial maturation, tissue integrity, apical-basal polarity
- MeSH terms
- Animals, Base Sequence, Cytoskeletal Proteins, Epithelium, Intercellular Junctions, Intestines, Trans-Activators, Cadherins, Caenorhabditis elegans, DNA Primers, Caenorhabditis elegans Proteins, beta Catenin
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