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Cited by 155Developmental Biology and Gene Regulationmelanin and skin pigmentationZebrafish Biomedical Research Applications

Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest

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Minchin, James E.N., Hughes, Simon M.·2008·Developmental Biology
DOI 10.1016/j.ydbio.2008.02.058PMC PMC3005709
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Abstract

The Pax3/7 gene family has a fundamental and conserved role during neural crest formation. In people, PAX3 mutation causes Waardenburg syndrome, and murine Pax3 is essential for pigment formation. However, it is unclear exactly how Pax3 functions within the neural crest. Here we show that pax3 is expressed before other pax3/7 members, including duplicated pax3b, pax7 and pax7b genes, early in zebrafish neural crest development. Knockdown of Pax3 protein by antisense morpholino oligonucleotides results in defective fate specification of xanthophores, with complete ablation in the trunk. Other pigment lineages are specified and differentiate. As a consequence of xanthophore loss, expression of pax7, a marker of the xanthophore lineage, is reduced in neural crest. Morpholino knockdown of Pax7 protein shows that Pax7 itself is dispensable for xanthophore fate specification, although yellow pigmentation is reduced. Loss of xanthophores after reduction of Pax3 correlates with a delay in melanoblast differentiation followed by significant increase in melanophores, suggestive of a Pax3-driven fate switch within a chromatophore precursor or stem cell. Analysis of other neural crest derivatives reveals that, in the absence of Pax3, the enteric nervous system is ablated from its inception. Therefore, Pax3 in zebrafish is required for specification of two specific lineages of neural crest, xanthophores and enteric neurons.

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  • Specification and differentiation of the third pigment cell type of zebrafish, the xanthophore, depends upon the paired-box transcription factors Pax3 and Pax7a/b.

    “Specification and differentiation of the third pigment cell type of zebrafish, the xanthophore, has been shown to depend upon the paired-box transcription factors Pax3 and Pax7a/b [59, 60].”

    From The MITF paralog tfec is required in neural crest development for fate specification of the iridophore lineage from a multipotent pigment cell progenitor (2021) · cites this paper as [59]

    The Discussion identifies Pax3 as required for xanthophore specification and links Pax7 reduction to later differentiation defects. It says Pax7 is dispensable for specification, while Pax7b’s role is probable rather than demonstrated. Thus the paper supports distinct roles for Pax3 and Pax7, but not an established dependence on both Pax7a and Pax7b. (LLM, conf=0.84)

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Authors
Minchin, J. E. N., & Hughes, S. M.
Affiliations
King's College London (GB)
Corresponding author
Hughes, Simon M.
ORCID
Venue
Developmental Biology
Publisher
Elsevier BV
Published
2008-05
Volume
317
Issue
2
Pages
508-522
ISSN
0012-1606 (ISSN-L)
DOI
10.1016/j.ydbio.2008.02.058
PMC
PMC3005709
OpenAlex
W2070314600
Cited by
155
References
0
Citations by year
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FWCI
2.79
Type
journal article
Language
en
Open access
Licence
Locations
  • Developmental Biology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • Research Portal (King's College London) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Keywords
Pax3, Pax7, neural crest, zebrafish, enteric nervous system, yellow pigmentation, morpholino knockdown
MeSH terms
PAX3 Transcription Factor, Animals, Bayes Theorem, Immunohistochemistry, Neural Crest, Oligonucleotides, Phylogeny, Pigments, Biological, Zebrafish, Blotting, Western, In Situ Hybridization, Enteric Nervous System, Zebrafish Proteins, Paired Box Transcription Factors, PAX7 Transcription Factor

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