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Cited by 181Developmental Biology and Gene RegulationNeurogenesis and neuroplasticity mechanismsCongenital gastrointestinal and neural anomalies

Analysis of early human neural crest development

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Betters, Erin, Liu, Ying, Kjaeldgaard, Anders, Sundström, Erik, García-Castro, Martín I.·2010·Developmental Biology
DOI 10.1016/j.ydbio.2010.05.012PMC PMC2927129
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Abstract

The outstanding migration and differentiation capacities of neural crest cells (NCCs) have fascinated scientists since Wilhelm His described this cell population in 1868. Today, after intense research using vertebrate model organisms, we have gained considerable knowledge regarding the origin, migration and differentiation of NCCs. However, our understanding of NCC development in human embryos remains largely uncharacterized, despite the role the neural crest plays in several human pathologies. Here, we report for the first time the expression of a battery of molecular markers before, during, or following NCC migration in human embryos from Carnegie Stages (CS) 12 to 18. Our work demonstrates the expression of Sox9, Sox10 and Pax3 transcription factors in premigratory NCCs, while actively migrating NCCs display the additional transcription factors Pax7 and AP-2alpha. Importantly, while HNK-1 labels few migrating NCCs, p75(NTR) labels a large proportion of this population. However, the broad expression of p75(NTR) - and other markers - beyond the neural crest stresses the need for the identification of additional markers to improve our capacity to investigate human NCC development, and to enable the generation of better diagnostic and therapeutic tools.

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  • Within the cell mass, there appeared to be two sub-types of CNCC-like cells expressing either the mesenchyme-associated marker PDGFRα or the neuro-glial-associated markers SOX10 and P75.

    “Within the cell mass, there appeared to be two sub-types of CNCC-like cells expressing either the mesenchyme-associated marker PDGFRα or the neuro-glial-associated markers SOX10 and P75 (Extended Data Fig. 2)15,16.”

    From Hair-bearing human skin generated entirely from pluripotent stem cells (2020) · cites this paper as [15]

    The Results (Carnegie Stage 12) report SOX10- and p75NTR-positive cells in cranial mesenchyme, supporting their use as neural crest markers. The paper does not establish a PDGFRα-positive subtype or two distinct CNCC-like subtypes within the cell mass described by the citing authors. (LLM, conf=0.84)

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References 111 · Cited by 181

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Authors
Betters, E., Liu, Y., Kjaeldgaard, A., Sundström, E., & García-Castro, M. I.
Affiliations
Yale University (US)
ORCID
Venue
Developmental Biology
Publisher
Elsevier BV
Published
2010-08
Volume
344
Issue
2
Pages
578-592
ISSN
0012-1606 (ISSN-L)
DOI
10.1016/j.ydbio.2010.05.012
PMC
PMC2927129
OpenAlex
W2091048750
Cited by
181
References
111
Citations by year
2026: 6 · 2025: 9 · 2024: 7 · 2023: 8 · 2022: 12 · 2021: 16 · 2020: 15 · 2019: 12 · 2018: 11 · 2017: 13 · 2016: 14 · 2015: 9 · 2014: 11 · 2013: 17 · 2012: 13
FWCI
4.82
Type
journal article
Language
en
Open access
Licence
Locations
  • Developmental Biology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Swedish Research Council
  • Stockholm's Sjukhem Foundation
  • Knut och Alice Wallenberg Foundation
  • Yale University
  • Yale University Developmental Biology Training Grant
  • NIH
  • State of Conecticut (RO1DE017914, 09SCAYALE45)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Health Sciences › Medicine › Surgery › Congenital gastrointestinal and neural anomalies
Keywords
neural crest cells, human embryo, neural crest migration, neural crest differentiation, molecular markers, SOX9, SOX10, Pax3, Pax7, AP-2α, HNK-1, p75 neurotrophin receptor, transcription factors
MeSH terms
Cell Differentiation, Humans, Neural Crest, Neurons, Transcription Factors, PAX7 Transcription Factor

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