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Cited by 42Pluripotent Stem Cells ResearchPregnancy and preeclampsia studiesDevelopmental Biology and Gene Regulation

ARID3A is required for mammalian placenta development

Open access
Rhee, Catherine, Edwards, Melissa, Dang, Christine, Harris, June, Brown, Mark, Kim, Jonghwan, Tucker, Haley O.·2017·Developmental Biology
DOI 10.1016/j.ydbio.2016.12.003PMC PMC5540318
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Abstract

Previous studies in the mouse indicated that ARID3A plays a critical role in the first cell fate decision required for generation of trophectoderm (TE). Here, we demonstrate that ARID3A is widely expressed during mouse and human placentation and essential for early embryonic viability. ARID3A localizes to trophoblast giant cells and other trophoblast-derived cell subtypes in the junctional and labyrinth zones of the placenta. Conventional Arid3a knockout embryos suffer restricted intrauterine growth with severe defects in placental structural organization. Arid3a null placentas show aberrant expression of subtype-specific markers as well as significant alteration in cytokines, chemokines and inflammatory response-related genes, including previously established markers of human placentation disorders. BMP4-mediated induction of trophoblast stem (TS)-like cells from human induced pluripotent stem cells results in ARID3A up-regulation and cytoplasmic to nuclear translocation. Overexpression of ARID3A in BMP4-mediated TS-like cells up-regulates TE markers, whereas pluripotency markers are down-regulated. Our results reveal an essential, conserved function for ARID3A in mammalian placental development through regulation of both intrinsic and extrinsic developmental programs.

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  • Five candidate TFs (ARID3A, C/EBPα, FOXA3, MYBL2, SOX13) are identified by overlapping TF enrichment and co-expression with GPC3.

    “By overlapping these lists, we identified five candidate TFs: ARID3A, C/EBPα, FOXA3, MYBL2, and SOX13, most of which are overexpressed in HCC and are implicated in hepatocarcinogenesis (Supplementary Fig. 6b, c)69–73.”

    From Multi-omic analysis of hepatocellular carcinoma reveals aberrant cis-regulatory changes and dysregulated retrotransposons with prognostic potential (2025) · cites this paper as [71]

    The five-TF list is presented in the citing sentence as the citing authors’ own identification, not as a finding attributed to this source. The source’s Results sections 6–7 examine ARID3A in trophoblast and placental development; searches for GPC3, the other listed TFs, and HCC found no passage addressing the citation-linked claim about overexpression or hepatocarcinogenesis. No page numbers were available in our copy. (LLM, conf=0.76)

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Cited by

Sorted by citations

Authors
Rhee, C., Edwards, M., Dang, C., Harris, J., Brown, M., Kim, J., & Tucker, H. O.
Affiliations
  • The University of Texas at Austin (US)
  • Colorado State University (US)
Corresponding author
Tucker, Haley O.
ORCID
Venue
Developmental Biology
Publisher
Elsevier BV
Published
2017-02
Volume
422
Issue
2
Pages
83-91
ISSN
0012-1606 (ISSN-L)
DOI
10.1016/j.ydbio.2016.12.003
PMC
PMC5540318
OpenAlex
W2559881236
Cited by
42
References
1
Citations by year
2026: 1 · 2025: 4 · 2024: 5 · 2023: 5 · 2022: 8 · 2021: 1 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 6
FWCI
1.99
Type
journal article
Language
en
Open access
Licence
Locations
  • Developmental Biology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • NIH (R01GM112722, R01CA31534)
  • CPRIT (RP120348, RP120459)
  • Marie Betzner Morrow Centennial Endowment
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
ARID3A, placental development, trophoblast giant cells, trophoblast differentiation, trophectoderm, BMP4, induced pluripotent stem cells, placental inflammation, fetal growth restriction, nuclear translocation
MeSH terms
Animals, Cell Differentiation, Cells, Cultured, DNA-Binding Proteins, Embryo, Mammalian, Female, Humans, Mice, Inbred C57BL, Morphogenesis, Placenta, Placentation, Pregnancy, Transcription Factors, Trophoblasts, Giant Cells, Cytokines, Mice, Knockout, Gene Expression Regulation, Developmental, Active Transport, Cell Nucleus, Mice, Bone Morphogenetic Protein 4, Induced Pluripotent Stem Cells

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