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Cited by 0Developmental Biology and Gene RegulationCell Adhesion Molecules ResearchCalcium Carbonate Crystallization and Inhibition

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CMTM4 is an adhesion modulator that regulates skeletal patterning and primary mesenchyme cell migration in sea urchin embryos

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Descoteaux, Abigail E., Radulovic, Marko, Alburi, Dona, Bradham, Cynthia A.·2025·Developmental Biology
DOI 10.1016/j.ydbio.2025.02.009

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Abstract

MARVEL proteins, including those of the CMTM gene family, are multi-pass transmembrane proteins that play important roles in vesicular trafficking and cell migration; however, little is understood about their role in development, and their role in skeletal patterning is unexplored. CMTM4 is the only CMTM family member found in the developmental transcriptome of the sea urchin Lytechinus variegatus. Here, we validate that LvCMTM4 is a transmembrane protein and show that perturbation of CMTM4 expression via zygotic morpholino or mRNA injection perturbs skeletal patterning, resulting in loss of secondary skeletal elements and rotational defects. We also demonstrate that normal levels of CMTM4 are required for normal PMC migration and filopodial organization, and that these effects are not due to gross mis-specification of the ectoderm. Finally, we show that CMTM4 is sufficient to mediate mesenchymal cell-cell adhesion. Taken together, these data suggest that CMTM4 controls PMC migration and biomineralization via adhesive regulation during sea urchin skeletogenesis. This is the first discovery of a functionally required adhesive gene in this skeletal patterning system.

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Metadata

Authors
Descoteaux, A. E., Radulovic, M., Alburi, D., & Bradham, C. A.
Affiliations
Boston University (US)
Corresponding author
Bradham, Cynthia A.
ORCID
Venue
Developmental Biology
Publisher
Elsevier BV
Published
2025-05
Volume
521
Pages
85-95
ISSN
0012-1606 (ISSN-L)
DOI
10.1016/j.ydbio.2025.02.009
OpenAlex
W4407367762
Cited by
0
References
4
FWCI
0.00
Type
journal article
Language
en
Open access
Licence
Locations
  • Developmental Biology (acceptedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion)
  • OpenBU (Boston University) (submittedVersion, cc-by-nc)
Funders
  • NIH National Institute of General Medical Sciences (1R35GM152180)
  • U.S. Department of Defense (HQ00342110008)
  • Boston University
  • National Science Foundation (IOS 1656752)
  • National Science Foundation Division of Integrative Organismal Systems
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Physical Sciences › Materials Science › Biomaterials › Calcium Carbonate Crystallization and Inhibition
Keywords
cell-cell adhesion, skeletal patterning, biomineralization, Lytechinus variegatus, filopodia, mesenchymal cells, morpholino knockdown
MeSH terms
Animals, Cell Adhesion, Cell Movement, Embryo, Nonmammalian, Mesoderm, Sea Urchins, Gene Expression Regulation, Developmental, Body Patterning, Lytechinus, MARVEL Domain-Containing Proteins

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CoChat. (2026). Citation check: CMTM4 is an adhesion modulator that regulates skeletal patterning and primary mesenchyme cell migration in sea urchin embryos. https://app.cochat.ai/papers/10.1016/j.ydbio.2025.02.009
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Descoteaux, A. E., Radulovic, M., Alburi, D., & Bradham, C. A. (2025). CMTM4 is an adhesion modulator that regulates skeletal patterning and primary mesenchyme cell migration in sea urchin embryos. Developmental Biology. https://doi.org/10.1016/j.ydbio.2025.02.009