The DOI matches its Crossref record; 36 of the 37 references it cites were found in a registry or an index.
A comparison of the tube forming potentials of early and late endothelial progenitor cells
Abstract
The identification of circulating endothelial progenitor cells (EPCs) has revolutionized approaches to cell-based therapy for injured and ischemic tissues. However, the mechanisms by which EPCs promote the formation of new vessels remain unclear. In this study, we obtained early EPCs from human peripheral blood and late EPCs from umbilical cord blood. Human umbilical vascular endothelial cells (HUVECs) were also used. Cells were evaluated for their tube-forming potential using our novel in vitro assay system. Cells were seeded linearly along a 60 mum wide path generated by photolithographic methods. After cells had established a linear pattern on the substrate, they were transferred onto Matrigel. Late EPCs formed tubular structures similar to those of HUVECs, whereas early EPCs randomly migrated and failed to form tubular structures. Moreover, late EPCs participate in tubule formation with HUVECs. Interestingly, late EPCs in Matrigel migrated toward pre-existing tubular structures constructed by HUVECs, after which they were incorporated into the tubules. In contrast, early EPCs promote sprouting of HUVECs from tubular structures. The phenomena were also observed in the in vivo model. These observations suggest that early EPCs cause the disorganization of pre-existing vessels, whereas late EPCs constitute and orchestrate vascular tube formation.
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“Even though these cells formed less orbicular and fewer tubules compared to batch 1 HBMECs, it was unlikely that they were early endothelial progenitor cells, as they established tubular structures on Matrigel [33].”
From Donor Variability Alters the Characteristics of Human Brain Microvascular Endothelial Cells (2025) · cites this paper as [33]
In the citing paper
“together, the assays robustly assess the health of ECs.”
From An inflammatory aging clock (iAge) based on deep learning tracks multimorbidity, immunosenescence, frailty and cardiovascular aging (2021) · cites this paper as [54]
Citations
Cites
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Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization
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Cited by
Sorted by citations
In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract
Arnaoutova, I., & Kleinman, H. K. (2010) · Nature Protocols · doi:10.1038/nprot.2010.6 · Cited by 760
Kolluru, G. K., Bir, S. C., & Kevil, C. G. (2012) · International Journal of Vascular Medicine · doi:10.1155/2012/918267 · Cited by 752
“They play an important role in angiogenesis as they proliferate, migrate and differentiate, and are a source for proangiogenic cytokines [232].”
BackgroundSayed, N., Huang, Y., Nguyen, K., Krejciova-Rajaniemi, Z., Grawe, A. P., Gao, T., Tibshirani, R., Hastie, T., Alpert, A., Cui, L., et al. (2021) · Nature Aging · doi:10.1038/s43587-021-00082-y · Cited by 572
Matrigel: From discovery and ECM mimicry to assays and models for cancer research
Benton, G., Arnaoutova, I., George, J., Kleinman, H. K., & Koblinski, J. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.06.005 · Cited by 477
Arnaoutova, I., George, J., Kleinman, H. K., & Benton, G. (2009) · Angiogenesis · doi:10.1007/s10456-009-9146-4 · Cited by 454
Metadata
- Authors
- Mukai, N., Akahori, T., Komaki, M., Li, Q., Kanayasu-Toyoda, T., Ishii-Watabe, A., Kobayashi, A., Yamaguchi, T., Abe, M., Amagasa, T., & Morita, I.
- Affiliations
- Tokyo Medical and Dental University (JP)
- National Institute of Health Sciences (JP)
- Corresponding author
- Morita, Ikuo
- Venue
- Experimental Cell Research
- Publisher
- Elsevier BV
- Published
- 2008-02
- Volume
- 314
- Issue
- 3
- Pages
- 430-440
- ISSN
- 0014-4827 (ISSN-L)
- OpenAlex
- W2084413357
- Cited by
- 157
- References
- 37
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 1 · 2023: 4 · 2022: 6 · 2021: 10 · 2020: 8 · 2019: 3 · 2018: 8 · 2017: 11 · 2016: 8 · 2015: 8 · 2014: 15 · 2013: 9 · 2012: 16
- FWCI
- 4.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Experimental Cell Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (KH31031)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Keywords
- endothelial progenitor cells, tube formation, angiogenesis, endothelial cell migration, sprouting angiogenesis, umbilical cord blood, peripheral blood, human umbilical vein endothelial cells, cell therapy
- MeSH terms
- Age Factors, Biological Assay, Cell Differentiation, Cells, Cultured, Collagen, Drug Combinations, Fluorescent Dyes, Humans, Infant, Newborn, Laminin, Microscopy, Electron, Organoids, Photochemistry, Proteoglycans, Stem Cells, Time Factors, Titanium, Neovascularization, Physiologic, Cell Culture Techniques, Tissue Engineering, Organogenesis, Endothelial Cells
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