Effects of transportation, transport medium and re-housing on Xenopus laevis (Daudin)
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Understanding the immediate and longer-term effects of transportation and re-housing in a laboratory species is crucial in order to refine the transfer process, enable the optimal introduction of new animals to a novel environment and to provide a sufficient acclimatisation period before usage. Whilst consideration of animal welfare in most model vertebrate species has received attention, little quantitative evidence exists for the optimal care of the common laboratory amphibian Xenopus laevis. Techniques for the non-invasive welfare assessment of amphibians are also limited and here a non-invasive physiological assay was developed to investigate the impacts of transportation, transport medium and re-housing on X. laevis. First the impacts of transportation and transport medium (water, damp sponge or damp sphagnum moss) were investigated. Transportation caused an increase in water-borne corticosterone regardless of transport medium. Frogs transported in damp sphagnum moss also had a greater decrease in body mass in comparison to frogs not transported, suggesting that this is the least suitable transport medium for X. laevis. Next the prolonged impacts of transportation and re-housing were investigated. Frogs were transported between research facilities with different housing protocols. Samples were collected prior to and immediately following transportation, as well as 1 day, 7 days and 35 days after re-housing. Water-borne corticosterone increased following transportation an…
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Physiological acclimatisation after a stressful event, such as transport, but before the experiment begins allows stabilisation of physiological responses of the animal.
“Physiological acclimatisation after a stressful event, such as transport (e.g., between supplier, animal facility, operating theatre, and laboratory), but before the experiment begins allows stabilisation of physiological responses of the animal [137,138].”
From Reporting animal research: Explanation and elaboration for the ARRIVE guidelines 2.0 (2020) · cites this paper as [137]
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Reporting animal research: Explanation and elaboration for the ARRIVE guidelines 2.0
Percie du Sert, N., Ahluwalia, A., Alam, S., Avey, M. T., Baker, M., Browne, W. J., Clark, A., Cuthill, I. C., Dirnagl, U., Emerson, M., et al. (2020) · PLOS Biology · doi:10.1371/journal.pbio.3000411 · Cited by 2,677 · see citing works
- Authors
- Holmes, A. M., Emmans, C. J., Coleman, R., Smith, T. E., & Hosie, C. A.
- Affiliations
- University of Chester (GB)
- Corresponding author
- Hosie, Charlotte A.
- Venue
- General and Comparative Endocrinology
- Publisher
- Elsevier BV
- Published
- 2018-09
- Volume
- 266
- Pages
- 21-28
- ISSN
- 0016-6480 (ISSN-L)
- OpenAlex
- W2790588329
- Cited by
- 16
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 1
- FWCI
- 2.56
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- General and Comparative Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- ChesterRep (University of Chester) (submittedVersion, cc-by-nc-nd)
- Funders
- NC3Rs (NC/K000497/1)
- Topics
- Health Sciences › Veterinary › Small Animals › Animal Behavior and Welfare Studies
- Health Sciences › Veterinary › Small Animals › Animal testing and alternatives
- Physical Sciences › Environmental Science › Global and Planetary Change › Amphibian and Reptile Biology
- Keywords
- Xenopus laevis, animal transport, transport media, corticosterone, stress response, weight loss, acclimatization
- MeSH terms
- Acclimatization, Animals, Animal Welfare, Corticosterone, Female, Housing, Animal, Male, Transportation, Water, Xenopus laevis
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