Microsatellite instability, promoter methylation and protein expression of the DNA mismatch repair genes in epithelial ovarian cancer
Open accessAbstract
The role of defective mismatch repair (MMR) system in ovarian carcinoma is not well defined. The purpose of the study was to determine the relationship between microsatellite instability (MSI), promoter methylation and protein expression of MMR genes in epithelial ovarian carcinoma (EOC). MSI and promoter methylation of MLH1, MSH2 and PMS2 genes were studied using PCR methods in the study cohort. A small subset of samples was used to analyze the protein expression by immunohistochemistry (IHC). MSI was observed in >60% of tumor samples and 47% of normal ovaries. MLH1 was methylated in 37.5% and 64.3% EOCs and LMP tumors. The loss of immunoexpression of MMR genes was not seen in ovarian tumors. There was no correlation between MSI, promoter methylation and protein expression of the MMR genes suggesting that each may function independently. MSI is a common event in ovarian carcinoma and may increase the clinical awareness of the subset of tumors.
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Microsatellite instability (MSI) was reported in leukemia, gastric, ovarian, breast, and colon cancers.
“Microsatellite instability (MSI) was reported in leukemia [25], gastric [26], ovarian [27], breast [28], and colon cancers [29].”
From Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines (2017) · cites this paper as [27]
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Research works printed but not linked: 2
- T. Pal, J. Permuth-Wey, T.A. Sellers, A review of the clinical relevance of mismatch- repair deficiency in ovarian cancer, Cancer 113 (2008) 733-742.
- A.M. Megan, W. Nicolas, Frequency of mismatch repair deficiency in ovarian cancer: a systematic review, Int. J. Cancer 129 (8) (2011) 1914-1922.
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Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines
Huang, Y., Liu, Y., Zheng, C., & Shen, C. (2017) · PLOS ONE · doi:10.1371/journal.pone.0170384 · Cited by 105 · see citing works
- Authors
- V., S., Bhagat, R., C.S., P., V.R., P., & Krishnamoorthy, L.
- Affiliations
- Kidwai Memorial Institute of Oncology (IN)
- Corresponding author
- Krishnamoorthy, Lakshmi
- Venue
- Genomics
- Publisher
- Elsevier BV
- Published
- 2014-10
- Volume
- 104
- Issue
- 4
- Pages
- 257-263
- ISSN
- 0888-7543 (ISSN-L)
- OpenAlex
- W2067210754
- Cited by
- 31
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 6 · 2022: 3 · 2020: 2 · 2019: 1 · 2018: 4 · 2017: 3 · 2016: 7 · 2015: 1
- FWCI
- 2.72
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Genomics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Mental Health and Neurosciences
- Indian Council of Medical Research
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
- Health Sciences › Medicine › Reproductive Medicine › Ovarian cancer diagnosis and treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › BRCA gene mutations in cancer
- Keywords
- microsatellite instability, promoter methylation, DNA mismatch repair, epithelial ovarian cancer, MLH1, MSH2, PMS2, protein expression, DNA methylation, Lynch syndrome, tumor suppressor
- MeSH terms
- MutL Protein Homolog 1, Mismatch Repair Endonuclease PMS2, Adenosine Triphosphatases, Carcinoma, DNA-Binding Proteins, Female, Humans, Nuclear Proteins, Ovarian Neoplasms, Promoter Regions, Genetic, Microsatellite Repeats, DNA Methylation, Genomic Instability, DNA Repair Enzymes, Adaptor Proteins, Signal Transducing, MutS Homolog 2 Protein, DNA Mismatch Repair
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