The DOI matches its Crossref record; 43 of the 44 references it cites were found in a registry or an index.
Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism
Abstract
The concept of "photosynthetic biofuels" envisions application of a single organism, acting both as photo-catalyst and producer of ready-made fuel. This concept was applied upon genetic engineering of the cyanobacterium Synechocystis, conferring the ability to generate volatile isoprene hydrocarbons from CO(2) and H(2)O. Heterologous expression of the Pueraria montana (kudzu) isoprene synthase (IspS) gene in Synechocystis enabled photosynthetic isoprene generation in these cyanobacteria. Codon-use optimization of the kudzu IspS gene improved expression of the isoprene synthase in Synechocystis. Use of the photosynthesis psbA2 promoter, to drive the expression of the IspS gene, resulted in a light-intensity-dependent isoprene synthase expression. Results showed that oxygenic photosynthesis can be re-directed to generate useful small volatile hydrocarbons, while consuming CO(2), without a prior requirement for the harvesting, dewatering and processing of the respective biomass.
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In the citing paper
“In a recent study, Synechocystis PCC 6803 was genetically modified with the Pueraria montana ispS gene to enable the production of isoprene in this microorganism (Lindberg et al. 2010).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Lindberg et al. 2010
In the citing paper
“Previous work has shown that a minimum of the C derived from photosynthesis is directed to pathways involved in the biofuel production (Lindberg et al. 2010).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Lindberg et al. 2010
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Structural and Chemical Biology of Terpenoid Cyclases
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Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol
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Biofuel production: Challenges and opportunities
Rodionova, M. V., Poudyal, R. S., Tiwari, I., Voloshin, R. A., Zharmukhamedov, S. K., Nam, H. G., Zayadan, B. K., Bruce, B. D., Hou, H. J. M., & Allakhverdiev, S. I. (2016) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2016.11.125 · Cited by 766
Systems metabolic engineering of microorganisms for natural and non-natural chemicals
Lee, J. W., Na, D., Park, J. M., Lee, J., Choi, S., & Lee, S. Y. (2012) · Nature Chemical Biology · doi:10.1038/nchembio.970 · Cited by 736
Fuelling the future: microbial engineering for the production of sustainable biofuels
Liao, J. C., Mi, L., Pontrelli, S., & Luo, S. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.32 · Cited by 604
Metadata
- Authors
- Lindberg, P., Park, S., & Melis, A.
- Affiliations
- University of California, Berkeley (US)
- Corresponding author
- Melis, Anastasios
- Venue
- Metabolic Engineering
- Publisher
- Elsevier BV
- Published
- 2010-01
- Volume
- 12
- Issue
- 1
- Pages
- 70-79
- ISSN
- 1096-7176 (ISSN-L)
- OpenAlex
- W2032641946
- Cited by
- 596
- References
- 44
- Citations by year
- 2026: 19 · 2025: 17 · 2024: 19 · 2023: 29 · 2022: 34 · 2021: 42 · 2020: 39 · 2019: 21 · 2018: 44 · 2017: 40 · 2016: 57 · 2015: 53 · 2014: 57 · 2013: 51 · 2012: 38
- FWCI
- 9.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Metabolic Engineering (publishedVersion)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- cyanobacteria, Synechocystis, isoprene synthase, Pueraria lobata, codon optimization, heterologous expression, volatile hydrocarbons, oxygenic photosynthesis
- MeSH terms
- Bacterial Proteins, Butadienes, Pentanes, Photosynthesis, Plant Proteins, Promoter Regions, Genetic, Alkyl and Aryl Transferases, Pueraria, Photosystem II Protein Complex, Hemiterpenes, Synechocystis
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