Metabolic engineering of omega-3 long-chain polyunsaturated fatty acids in plants using an acyl-CoA Δ6-desaturase with ω3-preference from the marine microalga Micromonas pusilla
Abstract
Long-chain (> or = C20) polyunsaturated fatty acids (LC-PUFA) EPA and DHA (20:5(Delta5,8,11,14,17) and 22:6(Delta4,7,10,13,16,19)) have well-documented health benefits against coronary heart disease, rheumatoid arthritis and other disorders. Currently, the predominant sources of these fatty acids are marine fish and algal oils, but research is being conducted to ensure that a sustainable, land-based production system can be developed. We here describe the metabolic engineering of an artificial pathway that produces 26% EPA in leaf triacylglycerol using a newly-identified Delta6-desaturase from the marine microalga Micromonas pusilla. We also demonstrate that this enzyme appears to function as an acyl-CoA desaturase that has preference for omega3 substrates both in planta and in yeast. Phylogenetic analysis indicates that this desaturase shares highly conserved motifs with previously described acyl-CoA Delta6-desaturases.
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Diatom species have been identified that generate biomolecules with human health benefits or commercial application, such as omega-3 fatty acids.
“In addition, diatom species have been identified that generate a number of interesting biomolecules that have human health benefits or commercial application, such as omega-3 fatty acids [89,90].”
From Biofuels from algae: Challenges and potential (2010) · cites this paper as [89]
Successful experiments improving lipid composition and content in higher plants suggest that improving lipid production in algae is a tenable option to improve biofuel characteristics.
“These successful experiments suggest that improving lipid production in algae is a tenable option to improve biofuel charactieristics.”
From Biofuels from algae: Challenges and potential (2010) · cites this paper as [89]
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References 31 · Cited by 132
- Authors
- Petrie, J. R., Shrestha, P., Mansour, M. P., Nichols, P. D., Liu, Q., & Singh, S. P.
- Affiliations
- Commonwealth Scientific and Industrial Research Organisation (AU)
- CSIRO Oceans and Atmosphere (AU)
- Corresponding author
- Singh, Surinder P.
- Venue
- Metabolic Engineering
- Publisher
- Elsevier BV
- Published
- 2010-05
- Volume
- 12
- Issue
- 3
- Pages
- 233-240
- ISSN
- 1096-7176 (ISSN-L)
- OpenAlex
- W2060174959
- Cited by
- 132
- References
- 31
- Citations by year
- 2025: 6 · 2024: 2 · 2023: 6 · 2022: 3 · 2021: 6 · 2020: 8 · 2019: 9 · 2018: 4 · 2017: 6 · 2016: 9 · 2015: 8 · 2014: 11 · 2013: 12 · 2012: 19
- FWCI
- 7.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Metabolic Engineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- omega-3 fatty acids, long-chain polyunsaturated fatty acids, EPA, docosahexaenoic acid, Micromonas pusilla, triglycerides, plant lipid metabolism, phylogenetic analysis, yeast expression system
- MeSH terms
- Chlorophyta, Animals, Biochemical Phenomena, Docosahexaenoic Acids, Fatty Acids, Fatty Acids, Unsaturated, Fishes, Plants, Saccharomyces cerevisiae, Stearoyl-CoA Desaturase, Fatty Acids, Omega-3, Fatty Acid Desaturases, Eukaryota
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