The DOI matches its Crossref record; 93 of the 93 references it cites were found in a registry or an index.
Cell-free synthetic biology: Thinking outside the cell
Open accessAbstract
Cell-free synthetic biology is emerging as a powerful technology aimed to understand, harness, and expand the capabilities of natural biological systems without using intact cells. Cell-free systems bypass cell walls and remove genetic regulation to enable direct access to the inner workings of the cell. The unprecedented level of control and freedom of design, relative to in vivo systems, has inspired the rapid development of engineering foundations for cell-free systems in recent years. These efforts have led to programmed circuits, spatially organized pathways, co-activated catalytic ensembles, rational optimization of synthetic multi-enzyme pathways, and linear scalability from the micro-liter to the 100-liter scale. It is now clear that cell-free systems offer a versatile test-bed for understanding why nature’s designs work the way they do and also for enabling biosynthetic routes to novel chemicals, sustainable fuels, and new classes of tunable materials. While challenges remain, the emergence of cell-free systems is poised to open the way to novel products that until now have been impractical—if not impossible—to produce by other means.
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Cites
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Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Gibson, D. G., Glass, J. I., Lartigue, C., Noskov, V. N., Chuang, R.-Y., Algire, M. A., Benders, G. A., Montague, M. G., Ma, L., Moodie, M. M., et al. (2010) · Science · doi:10.1126/science.1190719 · Cited by 2,638
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Cited by
Cell-free gene expression: an expanded repertoire of applications
Silverman, A. D., Karim, A. S., & Jewett, M. C. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-019-0186-3 · Cited by 593
Self-citationMoses, T., Pollier, J., Thevelein, J. M., & Goossens, A. (2013) · New Phytologist · doi:10.1111/nph.12325 · Cited by 245
Reconstituting Natural Cell Elements in Synthetic Cells
Gaut, N. J., & Adamala, K. P. (2021) · Advanced Biology · doi:10.1002/adbi.202000188 · Cited by 92
Ayoubi‐Joshaghani, M. H., Dianat‐Moghadam, H., Seidi, K., Jahanban‐Esfahalan, A., Zare, P., & Jahanban‐Esfahlan, R. (2020) · Biotechnology and Bioengineering · doi:10.1002/bit.27248 · Cited by 52
Metadata
- Authors
- Hodgman, C. E., & Jewett, M. C.
- Venue
- Metabolic Engineering
- Publisher
- Elsevier BV
- Published
- 2012-05
- Volume
- 14
- Issue
- 3
- Pages
- 261-269
- ISSN
- 1096-7176 (ISSN-L)
- OpenAlex
- W1963611162
- Cited by
- 425
- References
- 93
- FWCI
- 9.87
- Type
- journal article
- Language
- en
- Open access
- green
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Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
