The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites
Open accessAbstract
Control of native enzyme levels is important when optimizing strains for overproduction of heterologous compounds. However, for many central metabolic enzymes, static knockdown results in poor growth and protein expression. We have developed a strategy for dynamically modulating the abundance of native enzymes within the host cell and applied this to a model system for myo-inositol production from glucose. This system relies on controlled degradation of a key glycolytic enzyme, phosphofructokinase-I (Pfk-I). Through tuning Pfk-I levels, we have been able to develop an E. coli strain with a growth mode close to wild type and a production mode with an increased glucose-6-phosphate pool available for conversion into myo-inositol. The switch to production mode is trigged by inducer addition, allowing yield, titer, and productivity to be managed through induction time. By varying the time of Pfk-I degradation, we were able to achieve a two-fold improvement in yield and titers of myo-inositol.
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Cites
Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli
Bennett, B. D., Kimball, E. H., Gao, M., Osterhout, R., Van Dien, S. J., & Rabinowitz, J. D. (2009) · Nature Chemical Biology · doi:10.1038/nchembio.186 · Cited by 1,830
The Moderately Efficient Enzyme: Evolutionary and Physicochemical Trends Shaping Enzyme Parameters
Bar-Even, A., Noor, E., Savir, Y., Liebermeister, W., Davidi, D., Tawfik, D. S., & Milo, R. (2011) · Biochemistry · doi:10.1021/bi2002289 · Cited by 1,050
Cited by
Metabolic Burden: Cornerstones in Synthetic Biology and Metabolic Engineering Applications
Wu, G., Yan, Q., Jones, J. A., Tang, Y. J., Fong, S. S., & Koffas, M. A. G. (2016) · Trends in Biotechnology · doi:10.1016/j.tibtech.2016.02.010 · Cited by 666
Gupta, A., Reizman, I. M. B., Reisch, C. R., & Prather, K. L. J. (2017) · Nature Biotechnology · doi:10.1038/nbt.3796 · Cited by 490
Self-citationSynthetic biology to access and expand nature's chemical diversity
Smanski, M. J., Zhou, H., Claesen, J., Shen, B., Fischbach, M. A., & Voigt, C. A. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2015.24 · Cited by 481
Optogenetic regulation of engineered cellular metabolism for microbial chemical production
Zhao, E. M., Zhang, Y., Mehl, J., Park, H., Lalwani, M. A., Toettcher, J. E., & Avalos, J. L. (2018) · Nature · doi:10.1038/nature26141 · Cited by 355
Lu, H., Yadav, V., Bilal, M., & Iqbal, H. M. N. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2021.132574 · Cited by 96
Metadata
- Authors
- Brockman, I. M., & Prather, K. L. J.
- Venue
- Metabolic Engineering
- Publisher
- Elsevier BV
- Published
- 2015-03
- Volume
- 28
- Pages
- 104-113
- ISSN
- 1096-7176 (ISSN-L)
- OpenAlex
- W2005454774
- Cited by
- 164
- References
- 3
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- bronze
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