Identification of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent its occurrence during vector purification and formulation

Wright, J. Fraser, Le, Tannie, Prado, Joseph, Bahr-Davidson, Jennifer, Smith, Peter H., Zhen, Zhu, Sommer, Jurg M., Pierce, Glenn F. +1 more·2005·Molecular Therapy

Cited by (157)

Items 101–157 of 157

  1. In-Depth Comparison of Adeno-Associated Virus Containing Fractions after CsCl Ultracentrifugation Gradient Separation
    Mojca Janc, Kaja Zevnik, Ana Dolinar, Tjaša Jakomin, Maja Štalekar, Katarina Bačnik, Denis Kutnjak, Magda Tušek Žnidarič, Lorena Zentilin, Dmitrii Fedorov et al. · 2024 · Viruses
  2. Optimization of adenoviral production in human embryonic kidney cells using response surface methodology
    Chi‐Hsien Liu, Pei‐Shan Wu · 2007 · Journal of Bioscience and Bioengineering
  3. Calcium-ion-modulated ceramic hydroxyapatite resin for the scalable purification of recombinant Adeno-Associated Virus serotype 9
    Weihong Qu, Mingxi Wang, Yaqing Wu, Yinghui Lv, Wang Qizhao, Ruian Xu · 2015 · Journal of Chromatography B
  4. Adeno-Associated Virus as Gene Delivery Vehicle into the Retina
    Shuyun Deng, Kazuhiro Oka · 2019 · Methods in molecular biology
  5. Enrichment of Full AAV2 Using Multicolumn Countercurrent Solvent Gradient Purification (MCSGP)
    Julia M. Müller, Tereza Müllerová, Daniela Tobler, Damian Hauri, Ralf Plieninger, Yuki Higuchi, Ryosuke Takahashi, Sebastian Vogg, Thomas Müller‐Späth, Thomas K. Villiger · 2025 · Biotechnology and Bioengineering
  6. Approaches to purification and concentration of rAAV vectors for gene therapy
    Lyubava Belova, Konstantin S. Kochergin-Nikitsky, Anastasia Erofeeva, А. В. Лавров, Svetlana Smirnikhina · 2022 · BioEssays
  7. Bioengineering strategies for gene delivery
    Shahin Shams, Eduardo Alexandre Silva · 2019 · Elsevier eBooks
  8. Membrane Filtration of Nanoscale Biomaterials: Model System and Membrane Performance Evaluation for AAV2 Viral Vector Clarification and Recovery
    Mara Leach, Kearstin L. Edmonds, Emily Ingram, Rebecca Dutch, Sumith Ranil Wickramasinghe, Malgorzata Chwatko, Dibakar Bhattacharyya · 2025 · Nanomaterials
  9. Comprehensive stress study on recombinant adeno-associated virus vectors: Evaluating the capabilities of established analytical techniques
    Jakob Heckel, Lukas Bongers, David Naylor, Ole Schmidt, Raphael Ruppert, Marco Thomann, Florian Semmelmann, Angie Kirchner, Alexandra H.E. Machado, Markus Haindl et al. · 2026 · Journal of Pharmaceutical Sciences
  10. Evaluation of Host Cell Impurity Effects on the Performance of Sterile Filtration Processes for Therapeutic Viruses
    Evan Wright, Karina Kawka, Maria Fe C. Medina, David R. Latulippe · 2022 · Membranes
  11. Studying the ssDNA loaded adeno-associated virus aggregation using coarse-grained molecular dynamics simulations
    Tibo Duran, Shivangi Naik, Leila Sharifi, Willow R. DiLuzio, Arani Chanda, Bodhisattwa Chaudhuri · 2024 · International Journal of Pharmaceutics
  12. Impact of salt on AAV8 capsid aggregation with single-stranded DNA: insights from coarse-grained molecular dynamics simulations
    Leila Sharifi, Jahan Bakhsh Ghasemi, Tibo Duran, Shivangi Naik, Willow R. DiLuzio, Amir Hossein Sharifi, Arani Chanda, Bodhisattwa Chaudhuri · 2025 · International Journal of Pharmaceutics
  13. Impact of electrostatics on the aggregation, genome release, and self-interactions of AAV9 capsids
    Lily Motabar, Veerabhadraiah Palakollu, Hassan Shahfar, Justin C. Thomas, Garrett Daniels, Adnan Arnaout, Jorge Francisco Haller, Christopher J. Roberts · 2025 · Journal of Pharmaceutical Sciences
  14. Inverted Quasi‐Spherical Droplets on Polydopamine–TiO2 Substrates for Enhancing Gene Delivery
    Seung‐Hyun Kim, Mihyun Lee, Mira Cho, Il‐Sun Kim, Kook In Park, Haeshin Lee, Jae‐Hyung Jang · 2017 · Macromolecular Bioscience
  15. Expression and purification of the recombinant HBcAg core particles derived from methyltrophic Pichia pastoris, and TEM and AFM of the core particles and their natural aggregates
    Hong-bao Chen · 2007 · Journal of Electron Microscopy
  16. Delivery of AAV for Expression of Fluorescent Biosensors in Juvenile Mouse Hippocampus
    Carlos Díaz-García, Nidhi Nathwani, Juan Ramón Martínez‐François, Gary Yellen · 2021 · BIO-PROTOCOL
  17. Adeno-associated virus serotype 9 structural heterogeneity and stability characterized by charge detection mass spectrometry
    Zachary M. Miller, Li F. Lin, David V. Schaffer, Evan R. Williams · 2025 · Molecular Therapy — Methods & Clinical Development
  18. Development of an optimized SEC method for characterization of genome DNA leakage from adeno-associated virus products
    Shuai Li, Xiaoyan Wang, Kuan-Yu Lai, Jonathan Wert, Li Zhi, Mohammed Shameem, Dingjiang Liu · 2024 · Analytical and Bioanalytical Chemistry
  19. Separation of empty and full adeno-associated viral capsids by MCSGP: From model-based design to implementation
    Luca Ossi, Helena Marie, Michael Schulte, Mattia Sponchioni · 2025 · Journal of Chromatography A
  20. Manufacturing Considerations and Challenges for AAV Ocular Gene Therapy
    Gerard A. Rodrigues, Evgenyi Shalaev, Thomas K. Karami, James Cunningham, Hongwen M. Rivers, Shaoxin Feng, Dinen Shah, Nigel K.H. Slater · 2021 · AAPS advances in the pharmaceutical sciences series
  21. Glycerol facilitates the disaggregation of recombinant adeno-associated virus serotype 2 on mica surface
    Peng Wang, Hai Li, Haijun Yang, Hua Bing Wang, Jun Lu, Yi Zhang, Jun Hu · 2007 · Colloids and Surfaces B Biointerfaces
  22. Optimizing laboratory practices for recombinant adeno-associated viral vectors: impact of stress factors on vector stability
    Irini Skaripa-Koukelli, Ole Schmidt, Christiane Ketterer, Rene Lardenoije, Sarah Qutaishat, Alexandra H.E. Machado, Christoph Dürr, Joost van Haasteren, Pierre Goldbach, Lea Fuchsloch et al. · 2025 · AAPS Open
  23. A proof of concept study on the use of online two-dimensional SEC-UV-RPLC-MS method for the multi-attribute characterization of gene therapy products
    Megane K. Aebischer, Serge L. Rudaz, Davy Guillarme · 2025 · Analytica Chimica Acta
  24. Efficient AAV9 Purification Using a Single-Step AAV9 Magnetic Affinity Beads Isolation
    Kian Chuan Sia, Zhen Ying Fu, Siti Humairah Mohd Rodhi, Joan Hua Yi Yee, Kun Qu, Shu Uin Gan · 2024 · International Journal of Molecular Sciences
  25. Suspension Culture Production and Purification of Adeno-Associated Virus by Iodixanol Density Gradient Centrifugation for In Vivo Applications
    Kathryn K. Harris, Oleksandr Kondratov, Sergei Zolotukhin · 2024 · Journal of Visualized Experiments
  26. A Novel Method for Separating Full and Empty Adeno-Associated Viral Capsids Using Ultrafiltration
    Deepraj Sarmah, Scott M. Husson · 2024 · Membranes
  27. Stresses, Stabilization, and Recent Insights in Freezing of Biologics
    Rui Ming Fang, Pooja Sane, Israel Borges Sebastiao, Bakul S. Bhatnagar · 2023 · AAPS advances in the pharmaceutical sciences series
  28. A Spatial Interpolation Approach to Assign Magnetic Resonance Imaging-Derived Material Properties for Finite Element Models of Adeno-Associated Virus Infusion Into a Recurrent Brain Tumor
    Reed Chen, Julian A. Rey, İbrahim Sacit Tuna, David Tran, Malisa Sarntinoranont · 2024 · Journal of Biomechanical Engineering
  29. Developing a robust and scalable platform for AAV8 production
    André Nascimento, Tiago Q. Faria, Tiago Nunes, Joana Gomes Oliveira, José Mendes, António Roldão, Cristina Peixoto · 2025 · Journal of Biotechnology
  30. Improving Aggregation Control of Recombinant Adeno‐Associated Virus Serotype 2 (rAAV2) With Small Sugars and Ionic Salts
    Alexander Burns, Saurav Datta · 2025 · Biotechnology Journal
  31. Manufacturing of Viral Gene Therapies
    G. Anthony Ramirez, Mehdi Gasmi · 2021 · International Ophthalmology Clinics
  32. Formulation development and feasibility of AAV5 as a lyophilized drug product
    Stephanie K. Vargas, Farrokh Janabi Sharifi, Reinard Nambayan, Saeed Moshashaée, Teruna J. Siahaan · 2025 · Journal of Pharmaceutical Sciences
  33. Particle Analytics: Comparative Approaches for Analysis of Viral Vectors
    Irini Skaripa-Koukelli, Aurelia Raducanu, Joost van Haasteren, Juliana Albrecht, Philippe Ringler, Alexandra H.E. Machado · 2023 · Springer eBooks
  34. Can novel bioreactors improve the cost of goods of viral vectors?
    Christos Stamatis, Alex Chatel, Suzanne S. Farid · 2023 · Cell and Gene Therapy Insights
  35. Enrichment of full AAV capsids by preparative strong anion exchange chromatography
    Suriyasri Subramanian, Marcin Dembek, Nadia Auchus, Alistair R. Hines, Paul W. A. Devine, Åsa Hagner-McWhirter, Jean‐Luc Maloisel, Thomas A. Linke · 2025 · Biotechnology Progress
  36. Investigation of the colloidal stability of adeno-associated viral vectors and how it influences their chromatographic purification
    Rosella Prestia, Helena Marie, Michael Schulte, Mattia Sponchioni · 2025 · Separation Science and Technology
  37. Selectivity for full AAV capsids in affinity capture with camelid ligands
    Lukas Bongers, Linda Elise Franken, D. K. Hoch, Veronika E. Huber, Veronika Öttl, Elena B. Raaf, Jürgen J. Hubbuch, Roberto Falkenstein, A. Martínez · 2025 · Journal of Chromatography A
  38. Lyophilization of Adeno-Associated Virus Serotypes for Storage and Global Distribution
    Erin B. McGlinch, Haley E. Mudrick, Christopher H. Evans, Michael A. Barry · 2025 · Biomedicines
  39. Recent advances for the pharmaceutical production of highly attenuated poxviruses as viral vector platforms
    Linus G. Weber, Michael Werner Wolff · 2026 · Expert Review of Vaccines
  40. Systematic formulation optimisation to enhance buffer exchange recovery and storage stability for adeno-associated virus (AAV2) vectors
    Braulio Carrillo Sanchez, Raquel Fernández-García, Spyridon Gerontas, John E. Hales, Jonathan W. Aylott, Paul Anthony Dalby · 2025 · International Journal of Pharmaceutics
  41. DISPERSION AND FIXATION OF ADENO-ASSOCIATED VIRUS WITH GLUTARALDEHYDE FOR AFM STUDIES
    PENG WANG, Xinyan Wang, Haijun Yang, Junhong Lü · 2008 · Surface Review and Letters
  42. Phase-Appropriate Potency Assay Development for AAV-Based Gene Therapy Products
    Elaine E. Peters, Roland Pach, Shyamtanu Datta · 2023 · Springer eBooks
  43. Continuous Capture of recombinant AAV Particles Using Twin-Column CaptureSMB
    Julia M Mueller, Daniela Tobler, Jules Buehler, Damian Hauri, Ralf Plieninger, Sven Göbel, Efe Saygili, Yuki Higuchi, Ryosuke Takahashi, Sebastian Vogg et al. · 2026 · bioRxiv (Cold Spring Harbor Laboratory)
  44. PLGA Depots for Controlled Release of Bevacizumab.
    Rae Sung Chang · 2016 · Deep Blue (University of Michigan)
  45. Emerging Gene Therapy Approaches Under Clinical Investigation for Retinal Degenerative Diseases
    Benjamin Bakall, Kendra A. Klein, Katrina A Mears · 2020 · Topics in medicinal chemistry
  46. Mate selection: testing evolutionary hypotheses in a Hong Kong Chinese context
    Sammy K. Ho · 2004
  47. Filtration Principles and Techniques for Bioprocessing of Viral Vector-Based Therapeutics
    Laurens Vergauwen, Claire Scanlan, Ratish Krishnan, Santosh Kumar Pandey, David Loong, Amritanshu Bhushan · 2023 · Springer eBooks
  48. The Tiling Algorithm – A general method for structural characterization of accurate long DNA sequence reads: application to AAV genome sequences
    Robert E. Bruccoleri, David Rouleau, Celia Slater, Dimpal Lata, Christopher Phillion, Samuel Adjei, Kiran Adhikari, Serena Dollive · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
  49. An Automated Workflow Leveraging Machine Learning for Physical Titer Determination from Cryo-TEM Images of Adeno-Associated Virus Capsids
    Lilianna C. Gutierrez, Anne Skaja Robinson · 2025 · Chemical & Biomedical Imaging
  50. Assessment of Enzyme-Mediated Modulation of rAAV Vectors: Effects on Cellular Transduction and Capsid Stability
    Seongkyu Yoon, George Liang · 2026 · Research Square
  51. Characterization of rAAV vectors packaging in baculovirusinfected insect cells
    Magalie Penaud‐Budloo · 2018 · theses.fr (ABES)
  52. Adeno-Associated Virus Co-Precipitation with Extracellular Vesicles for Genome Editing in Rodent Embryo
    Petr Nickl, Maria Barbiera, Jacopo Zini, Tereza Nickl, Aki Ushiki, Michaela Vaškovičová, Jitka Neburková, Vojtech Dolejs, Michaela Šímová, Jana Balounová et al. · 2026 · Research Square
  53. Combined Plasmid Redesign and Transfection Optimization Significantly Increases Upstream AAV Titers While Maintaining Vector Quality and In Vivo Potency
    Shiliang Hu, Yinxing Chen, Carmen Wu, Wilhad Hans Reuter, June Deng, Nannan Jia, Noel Walsh, Amy Bastille, Thomas M. Edwards Edwards, Matthias Hebben et al. · 2026 · Microorganisms
  54. Mechanistic Insights into Poloxamer 188–Mediated Stabilization of AAV8 Capsids Using Coarse-Grained Molecular Dynamics
    Leila Sharifi, Jahanbakhsh Ghasemi, Leo Santisi, Shivangi Naik, Willow R. DiLuzio, Arani Chanda, Bodhisattwa Chaudhuri · 2026 · Pharmaceutical Research
  55. Analytical and functional characterization of next-generation therapeutics: ADCs, CAR-T cells, rAAVs, mRNA and VLPs
    Anurag Singh Rathore, Davy Guillarme, Alain Beck, Vineela Peruri, Shweta Dubey · 2026 · TrAC Trends in Analytical Chemistry
  56. Efficient Cell Removal and Impurity Reduction for Poxvirus Purification in a Single Step Alluvial Filtration
    Linus G. Weber, Caroline Stephan, Georgios Makris, Leander John, Ingo Jordan, Michael Werner Wolff · 2026 · Biotechnology and Bioengineering
  57. Assessing AAV stability on affinity resins and the effects of long contact times through extensive washing
    Lukas Bongers, Leon Clemenz, Jorge Navas Martinez, Melanie Weber, Jürgen Hubbuch, Roberto Falkenstein, A. Martínez · 2026 · Frontiers in Bioengineering and Biotechnology