Size-Dependent in Vivo Growth Potential of Adult Rat Hepatocytes
Open accessAbstract
The present study was performed to determine whether hepatocytes show a size-dependent growth in vivo using as a growth assay system, a retrorsine/partial hepatectomy model of dipeptidyl dipeptidase IV-deficient (DPPIV(-)) mutant Fischer rats. Nearly pure populations of small hepatocytes (SHs) and parenchymal hepatocytes (PHs) were prepared from DPPIV(+) rats. The same number of these SHs and PHs was transplanted into the liver of retrorsine-treated and two-thirds partial hepatectomized DPPIV(-) rats. At 21 days after transplantation, colonies derived from donor hepatocytes were detected as DPPIV(+) cells by enzyme histochemistry. SHs were approximately three times more proliferative than PHs (673 +/- 25 cells/colony versus 226 +/- 10 cells/colony, mean +/- SE). SHs were subfractionated by a fluorescence-activated cell sorter into SH-R2s and SH-R3s. SH-R3s showed a lower extent of granularity and autofluorescence, and a smaller size than SH-R2s that showed characteristics similar to PHs. The growth potential of SH-R3s assayed as above was approximately three times higher than that of SH-R2s (1,101 +/- 46 cells/colony versus 341 +/- 13 cells). These results indicate that the in vivo growth potential of hepatocytes is heterogeneous and is correlated with their size, and the extent of their granularity and autofluorescence.
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Small hepatocytes found in retrorsine-treated rat livers and small Thy1+ hepatocytes observed in rat livers following galactosamine-induced injury have robust regenerative properties.
“An interesting but less well understood population of hepatocytes are the small hepatocytes found in retrorsine-treated rat livers[138], [139], [140] and the small Thy1+ hepatocytes observed in rat livers following galactosamine-induced injury141 (Fig. 3F). These small hepatocytes were reported to have robust regenerative properties.”
From Distinct hepatocyte identities in liver homeostasis and regeneration (2023) · cites this paper as [140]
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Research works printed but not linked: 16
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Avril, A., Pichard, V., Bralet, M.-P., & Ferry, N. (2004) · Journal of Hepatology · doi:10.1016/j.jhep.2004.07.029 · Cited by 62 · see citing works
Baron, M. G., Purcell, W. M., Jackson, S. K., Owen, S. F., & Jha, A. N. (2012) · Ecotoxicology · doi:10.1007/s10646-012-0965-5 · Cited by 58 · see citing works
Distinct hepatocyte identities in liver homeostasis and regeneration
Chen, F., Schönberger, K., & Tchorz, J. S. (2023) · JHEP Reports · doi:10.1016/j.jhepr.2023.100779 · Cited by 30 · see citing works
- Authors
- Katayama, S., Tateno, C., Asahara, T., & Yoshizato, K.
- Affiliations
- Hiroshima University (JP)
- Corresponding author
- Yoshizato, Katsutoshi
- Venue
- The American Journal of Pathology
- Publisher
- Elsevier BV
- Published
- 2001-01
- Volume
- 158
- Issue
- 1
- Pages
- 97-105
- ISSN
- 0002-9440 (ISSN-L)
- PMC
- PMC1850269
- OpenAlex
- W1984921880
- Cited by
- 51
- References
- 0
- Citations by year
- 2023: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2013: 4 · 2012: 4
- FWCI
- 3.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- American Journal Of Pathology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Surgery › Organ Transplantation Techniques and Outcomes
- Health Sciences › Medicine › Hepatology › Hepatocellular Carcinoma Treatment and Prognosis
- Keywords
- hepatocyte size, hepatocyte heterogeneity, retrorsine, partial hepatectomy, hepatocyte transplantation, hepatocyte proliferation, granularity, autofluorescence
- MeSH terms
- Albumins, alpha-Fetoproteins, Animals, Cell Count, Cell Division, Clone Cells, Female, Keratins, Liver, Male, Phenotype, Rats, Inbred F344, Cell Transplantation, Dipeptidyl Peptidase 4, Hepatocytes, Cell Size, Rats
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