Up-regulation of PDCD4 in senescent human diploid fibroblasts
Abstract
Programmed cell death 4 (PDCD4) has a common MI domain sharing with death associated protein 5 (DAP5) and a component of eukaryotic translation initiation factor (eIF4G) complex and it might also work as a tumor suppressor. We could find that the message and product of Pdcd4 gene were up-regulated in senescent human diploid fibroblasts. In yeast two hybrid analysis, the C-terminal region of PDCD4 interacted with ribosomal protein S13 (RPS13), ribosomal protein L5 (RPL5), and TI-227H. In in vitro binding assay, RPS13, a component of 40S ribosome was stably bound to PDCD4. We also found that PDCD4 was localized to polysome fractions. We could pull out eIF4G with GST-PDCD4, but eIF4E did not interact with PDCD4. From these results, we could assume that PDCD4 might regulate the eIF4G-dependent translation through direct interactions with eIF4G and RPS13 in senescent fibroblasts.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
PDCD4 is upregulated in senescent human fibroblasts.
“Kang et al. reported that PDCD4 is upregulated in senescent human fibroblasts [47].”
From Control Mechanisms of the Tumor Suppressor PDCD4: Expression and Functions (2019) · cites this paper as [47]
The role of PDCD4 in the RB-pathway might be different depending on the context and cell types.
“The results indicate that the role of PDCD4 in the RB-pathway might be different depending on the context and cell types.”
From Control Mechanisms of the Tumor Suppressor PDCD4: Expression and Functions (2019) · cites this paper as [47]
The citation lists haven’t been fetched yet
References 26 · Cited by 57
- Authors
- Kang, M.-J., Ahn, H.-S., Lee, J.-Y., Matsuhashi, S., & Park, W.-Y.
- Affiliations
- Seoul National University (KR)
- Saga University (JP)
- Corresponding author
- Park, Woong-Yang
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2002-04
- Volume
- 293
- Issue
- 1
- Pages
- 617-621
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2000330197
- Cited by
- 57
- References
- 26
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 5 · 2022: 2 · 2021: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- PDCD4, cellular senescence, human diploid fibroblasts, eIF4G, eIF4E, ribosomal protein S13, RPL5, translational regulation, polysomes, protein-protein interaction, tumor suppressor, D-AP5
- MeSH terms
- Animals, Cells, Cultured, Cycloheximide, Fibroblasts, Gene Expression Regulation, HeLa Cells, Humans, Infant, Newborn, Male, Peptide Initiation Factors, Polyribosomes, Proteins, Rabbits, Recombinant Proteins, Ribosomal Proteins, Skin Physiological Phenomena, RNA-Binding Proteins, Cellular Senescence, Apoptosis, Eukaryotic Initiation Factor-4G, Apoptosis Regulatory Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
