Fasting activates the gene expression of UCP3 independent of genes necessary for lipid transport and oxidation in skeletal muscle
Open accessAbstract
Fasting triggers a complex array of adaptive metabolic and hormonal responses including an augmentation in the capacity for mitochondrial fatty acid (FA) oxidation in skeletal muscle. This study hypothesized that this adaptive response is mediated by increased mRNA of key genes central to the regulation of fat oxidation in human skeletal muscle. Fasting dramatically increased UCP3 gene expression, by 5-fold at 15 h and 10-fold at 40 h. However the expression of key genes responsible for the uptake, transport, oxidation, and re-esterification of FA remained unchanged following 15 and 40 h of fasting. Likewise there was no change in the mRNA abundance of transcription factors. This suggests a unique role for UCP3 in the regulation of FA homeostasis during fasting as adaptation to 40 h of fasting does not require alterations in the expression of other genes necessary for lipid metabolism.
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UCP3 mRNA levels were elevated after 15 h (~5-fold) and 40 h (~10-fold) of fasting in humans.
“UCP3 mRNA levels were also elevated after 15 h (∼5-fold) and 40 h (∼10-fold) of fasting in humans (38).”
From Prolonged fasting identifies skeletal muscle mitochondrial dysfunction as consequence rather than cause of human insulin resistance (2010) · cites this paper as [38]
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References 47 · Cited by 42
- Authors
- Tunstall, R. J., Mehan, K. A., Hargreaves, M., Spriet, L. L., & Cameron-Smith, D.
- Affiliations
- Deakin University (AU)
- University of Guelph (CA)
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2002-06
- Volume
- 294
- Issue
- 2
- Pages
- 301-308
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2012197573
- Cited by
- 42
- References
- 47
- Citations by year
- 2026: 1 · 2025: 3 · 2023: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2014: 3 · 2013: 1 · 2012: 2
- FWCI
- 2.40
- Type
- journal article
- Language
- en
- Open access
- green
- figshare.com
- Licence
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Figshare (submittedVersion, cc-by)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- fasting, UCP3, skeletal muscle, fatty acid oxidation, mitochondrial fatty acid oxidation, gene expression, lipid metabolism, fatty acid homeostasis, lipid transport, transcription factors, metabolic adaptation
- MeSH terms
- Uncoupling Protein 3, Adaptation, Physiological, Adult, Biological Transport, Blood Glucose, Carrier Proteins, Fasting, Fatty Acids, Nonesterified, Female, Gene Expression Regulation, Glycerol, Humans, Insulin, Ion Channels, Male, Oxidation-Reduction, Reference Values, RNA, Messenger, Time Factors, Transcription Factors, Muscle, Skeletal, Mitochondrial Proteins, Lipid Metabolism
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