Microarray analysis of peroxisome proliferator-activated receptor-γ induced changes in gene expression in macrophages
Abstract
We used a combination of expression microarray and Northern blot analyses to identify target genes for peroxisome proliferator-activated receptor (PPAR) gamma in RAW264.7 macrophages. PPARgamma natural ligand 15-deoxy-Delta(12,14) prostaglandin and synthetic ligands ciglitazone and rosiglitazone increased the expression of scavenger receptor CD36 and ATP-binding cassette transporter A1, as well as adipophilin (a lipid droplet coating protein involved in intracellular lipid storage and transport), calpain (a protease implicated in ABCA1 protein degradation), and ADAM8 (a disintegrin and metalloprotease protein involved in cell adhesion). These findings are relevant to understanding the effect of PPARgamma activation on gene expression and cognate pathways in macrophages.
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PLIN2 (adipophilin) is a known PPARγ target gene.
“adipophilin [perilipin 2 (PLIN2)], a well-known PPARg target”
PLIN2 is regulated by PPARγ in macrophages.
“It has been shown to be regulated by PPARg in macrophages (Hodgkinson and Ye, 2003).”
Cites
Chinetti, G., Lestavel, S., Bocher, V., Remaley, A. T., Neve, B., Torra, I. P., Teissier, E., Minnich, A., Jaye, M., Duverger, N., et al. (2001) · Nature Medicine · doi:10.1038/83348 · Cited by 1,170 · see citing works
The Peroxisome Proliferator-activated Receptor δ Promotes Lipid Accumulation in Human Macrophages
Vosper, H., Patel, L., Graham, T. L., Khoudoli, G. A., Hill, A., Macphee, C. H., Pinto, I., Smith, S. A., Suckling, K. E., Wolf, C. R., et al. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m108482200 · Cited by 268 · see citing works
The Metalloprotease Disintegrin ADAM8
Schlomann, U., Wildeboer, D., Webster, A., Antropova, O., Zeuschner, D., Knight, C. G., Docherty, A. J. P., Lambert, M., Skelton, L., Jockusch, H., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m203355200 · Cited by 171 · see citing works
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Escribano, L., Simón, A.-M., Gimeno, E., Cuadrado-Tejedor, M., López de Maturana, R., García-Osta, A., Ricobaraza, A., Pérez-Mediavilla, A., Del Río, J., & Frechilla, D. (2010) · Neuropsychopharmacology · doi:10.1038/npp.2010.32 · Cited by 235 · see citing works
Schubert, M., Becher, S., Wallert, M., Maeß, M. B., Abhari, M., Rennert, K., Mosig, A. S., Große, S., Heller, R., Grün, M., et al. (2020) · Molecular Pharmacology · doi:10.1124/mol.119.117887 · Cited by 35 · see citing works
- Authors
- Hodgkinson, C. P., & Ye, S.
- Affiliations
- University of Southampton (GB)
- Corresponding author
- Hodgkinson, Conrad P
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2003-08
- Volume
- 308
- Issue
- 3
- Pages
- 505-510
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W1978065594
- Cited by
- 51
- References
- 3
- Citations by year
- 2025: 1 · 2023: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2015: 4 · 2014: 3 · 2013: 2 · 2012: 2
- FWCI
- 1.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- Funders
- British Heart Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Paraoxonase enzyme and polymorphisms
- Keywords
- PPARγ, macrophages, gene expression, PPAR target genes, 15-deoxy-Δ12,14-prostaglandin J2, ciglitazone, rosiglitazone, CD36, ABCA1, adipophilin, calpain, ADAM8, lipid metabolism, RAW 264.7 macrophages
- MeSH terms
- Animals, Cell Line, Gene Expression Regulation, Macrophages, Models, Biological, Transcription Factors, Receptors, Cytoplasmic and Nuclear, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Lipid Metabolism
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