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Entropy and Heat Capacity of DNA Melting from Temperature Dependence of Single Molecule Stretching
Open accessAbstract
When a single molecule of double-stranded DNA is stretched beyond its B-form contour length, the measured force shows a highly cooperative overstretching transition. We have measured the force at which this transition occurs as a function of temperature. To do this, single molecules of DNA were captured between two polystyrene beads in an optical tweezers apparatus. As the temperature of the solution surrounding a captured molecule was increased from 11 degrees C to 52 degrees C in 500 mM NaCl, the overstretching transition force decreased from 69 pN to 50 pN. This reduction is attributed to a decrease in the stability of the DNA double helix with increasing temperature. These results quantitatively agree with a model that asserts that DNA melting occurs during the overstretching transition. With this model, the data may be analyzed to obtain the change in the melting entropy DeltaS of DNA with temperature. The observed nonlinear temperature dependence of DeltaS is a result of the positive change in heat capacity of DNA upon melting, which we determine from our stretching measurements to be DeltaC(p) = 60 +/- 10 cal/mol K bp, in agreement with calorimetric measurements.
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Cites
Predicting DNA duplex stability from the base sequence
Breslauer, K. J., Frank, R., Blöcker, H., & Marky, L. A. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.11.3746 · Cited by 1,837
Cited by
Optical tweezers in single-molecule biophysics
Bustamante, C. J., Chemla, Y. R., Liu, S., & Wang, M. D. (2021) · Nature Reviews Methods Primers · doi:10.1038/s43586-021-00021-6 · Cited by 615
Single-molecule experiments in biological physics: methods and applications
Ritort, F. (2006) · Journal of Physics: Condensed Matter · doi:10.1088/0953-8984/18/32/r01 · Cited by 393
van Mameren, J., Gross, P., Farge, G., Hooijman, P., Modesti, M., Falkenberg, M., Wuite, G. J. L., & Peterman, E. J. G. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0904322106 · Cited by 300
Quantifying how DNA stretches, melts and changes twist under tension
Gross, P., Laurens, N., Oddershede, L. B., Bockelmann, U., Peterman, E. J. G., & Wuite, G. J. L. (2011) · Nature Physics · doi:10.1038/nphys2002 · Cited by 257
Bianco, P., Bongini, L., Melli, L., Dolfi, M., & Lombardi, V. (2011) · Biophysical Journal · doi:10.1016/j.bpj.2011.06.039 · Cited by 28
Metadata
- Authors
- Williams, M. C., Wenner, J. R., Rouzina, I., & Bloomfield, V. A.
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 2001-04
- Volume
- 80
- Issue
- 4
- Pages
- 1932-1939
- ISSN
- 0006-3495 (ISSN-L)
- OpenAlex
- W2120110930
- Cited by
- 207
- References
- 1
- FWCI
- 3.37
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Williams, M. C., Wenner, J. R., Rouzina, I., & Bloomfield, V. A. (2001). Entropy and Heat Capacity of DNA Melting from Temperature Dependence of Single Molecule Stretching. Biophysical Journal. https://doi.org/10.1016/s0006-3495(01)76163-2
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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