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Ca2+ buffering and action potential-evoked Ca2+ signaling in dendrites of pyramidal neurons
Open accessAbstract
The effect of the fluorescent Ca2+ indicator dye Fura-2 on Ca2+ dynamics was studied in proximal apical dendrites of neocortical layer V and hippocampal CA1 pyramidal neurons in rat brain slices using somatic whole-cell recording and a charge-coupled device camera. A single action potential evoked a transient increase of intradendritic calcium concentration ([Ca2+]i) that was reduced in size and prolonged when the Fura-2 concentration was increased from 20 to 250 microM. Extrapolation to zero Fura-2 concentration suggests that "physiological" transients at 37 degrees C have large amplitudes (150-300 nM) and fast decays (time constant < 100 ms). Assuming a homogeneous compartment model for the dendrite, 0.5-1% of the total Ca2+ entering during an action potential was estimated to remain free. Washout of cytoplasmic Ca2+ buffers was not detectable, suggesting that they are relatively immobile. During trains of action potentials, [Ca2+]i increased and rapidly reached a steady state (time constant < 200 ms), fluctuating around a plateau level which depended linearly on the action potential frequency. Thus, the mean dendritic [Ca2+]i encodes the action potential frequency during physiological patterns of electrical activity and may regulate Ca(2+)-dependent dendritic functions in an activity-dependent way.
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“relative to the resting level of intracellular Ca2+ (20–50 nM) (109–111)”
From Synaptic memory and CaMKII (2023) · cites this paper as [111]
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Metadata
- Authors
- Helmchen, F., Imoto, K., & Sakmann, B.
- Affiliations
- Max Planck Society (DE)
- Max Planck Institute for Medical Research (DE)
- ORCID
- Helmchen, F.
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 1996-02
- Volume
- 70
- Issue
- 2
- Pages
- 1069-1081
- ISSN
- 0006-3495 (ISSN-L)
- PMC
- PMC1225009
- OpenAlex
- W2006602134
- Cited by
- 559
- References
- 47
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 2 · 2023: 9 · 2022: 13 · 2021: 9 · 2020: 11 · 2019: 14 · 2018: 18 · 2017: 12 · 2016: 18 · 2015: 27 · 2014: 23 · 2013: 17 · 2012: 27
- FWCI
- 10.02
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biophysical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Max Planck Digital Library (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- calcium buffering, dendritic calcium signaling, pyramidal neurons, hippocampal CA1, fura-2, calcium indicator dyes, action potential frequency
- MeSH terms
- Action Potentials, Animals, Biophysics, Buffers, Calcium, Cell Compartmentation, Dendrites, Fluorescent Dyes, Indicators and Reagents, Kinetics, Models, Neurological, Signal Transduction, Fura-2, Rats, Wistar, Pyramidal Cells, Rats, Biophysical Phenomena, In Vitro Techniques
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