Upregulation of intercellular adhesion molecule-1 expression on human endothelial cells by tumour necrosis factor-α in an in vitro model of the blood–brain barrier
Abstract
Adhesion molecules on the endothelial surface of the blood-brain barrier (BBB) play an important role in the pathogenesis of many encephalopathies, including multiple sclerosis (MS) and cerebral malaria (CM). The expression of four surface molecules of relevance to MS and CM on the immortalized human umbilical vein endothelial cell line, ECV304, was investigated using immunofluorescence flow cytometry. We found that ECV304 cells express intercellular adhesion molecule-1 (ICAM-1) and low levels of CD36, but not vascular cell adhesion molecule-1 (VCAM-1) or E-selectin. This expression pattern was unaltered on ECV304 cells which were co-cultured with C6 glioma cells; conditions under which the endothelial cells display enhanced barrier formation. Tumour necrosis factor-alpha (TNF-alpha), which is elevated in MS and CM, decreased the integrity of the barrier in co-cultured endothelial cells and upregulated the expression of ICAM-1 nine-fold. The significance of elevated ICAM-1 expression in relation to the binding of parasitised erythrocytes at the BBB in CM is discussed.
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In vitro stimulation of human brain endothelial cells with Aβ or TNF-α induced expression of selectin, ICAM-1, and VCAM-1, which are responsible for leukocyte adhesion and transport into the CNS.
“In vitro stimulating of human brain endothelial cells with Aβ or TNF-α induced the expression of endothelial adhesion molecules, including selectin, ICAM-1, and vascular cell adhesion protein 1 (VCAM-1), which are responsible for the adhesion and transport of leukocyte in to CNS.487,488”
From Role of neuroinflammation in neurodegeneration development (2023) · cites this paper as [488]
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- Authors
- Dobbie, M. S., Hurst, R. D., Klein, N. J., & Surtees, R. A. H.
- Affiliations
- University College London (GB)
- University of the West of England (GB)
- Corresponding author
- Hurst, Roger D.
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1999-06
- Volume
- 830
- Issue
- 2
- Pages
- 330-336
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2078276534
- Cited by
- 82
- References
- 0
- Citations by year
- 2025: 1 · 2023: 2 · 2022: 2 · 2021: 2 · 2019: 3 · 2018: 3 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 6
- FWCI
- 2.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Brain Research Trust
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- ICAM-1, TNF-α, blood-brain barrier, endothelial cells, ECV304 cells, CD36, VCAM-1, E-selectin, barrier integrity, multiple sclerosis, cerebral malaria, adhesion molecules
- MeSH terms
- Animals, Blood-Brain Barrier, Cell Line, Endothelium, Vascular, Flow Cytometry, Humans, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha, Up-Regulation, Intercellular Adhesion Molecule-1, CD36 Antigens, Vascular Cell Adhesion Molecule-1, E-Selectin, Rats
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