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A strategy for the generation of non‐aggregating mutants of Anthozoa fluorescent proteins
Abstract
Recently, we cloned several fluorescent proteins of different colors homologous to Aequorea victoria green fluorescent protein, which have great biotechnological potential as in vivo markers of gene expression. However, later investigations revealed severe drawbacks in the use of novel fluorescent proteins (FPs), in particular, the formation of tetramers (tetramerization) and high molecular weight aggregates (aggregation). In this report, we employ a mutagenic approach to resolve the problem of aggregation. The elimination of basic residues located near the N‐termini of FPs results in the generation of non‐aggregating versions of several FPs, specifically, drFP583 (DsRed), DsRed‐Timer, ds/drFP616, zFP506, zFP538, amFP486, and asFP595.
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Cited by
A monomeric red fluorescent protein
Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.082243699 · Cited by 2,365
Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues
Chudakov, D. M., Matz, M. V., Lukyanov, S., & Lukyanov, K. A. (2010) · Physiological Reviews · doi:10.1152/physrev.00038.2009 · Cited by 1,375
Self-citationTwo-photon absorption properties of fluorescent proteins
Drobizhev, M., Makarov, N. S., Tillo, S. E., Hughes, T. E., & Rebane, A. (2011) · Nature Methods · doi:10.1038/nmeth.1596 · Cited by 675
Fluorescent proteins as a toolkit for in vivo imaging
Chudakov, D. M., Lukyanov, S., & Lukyanov, K. A. (2005) · Trends in Biotechnology · doi:10.1016/j.tibtech.2005.10.005 · Cited by 498
Self-citationKindling fluorescent proteins for precise in vivo photolabeling
Chudakov, D. M., Belousov, V. V., Zaraisky, A. G., Novoselov, V. V., Staroverov, D. B., Zorov, D. B., Lukyanov, S., & Lukyanov, K. A. (2003) · Nature Biotechnology · doi:10.1038/nbt778 · Cited by 307
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Metadata
- Authors
- Yanushevich, Y. G., Staroverov, D. B., Savitsky, A. P., Fradkov, A. F., Gurskaya, N. G., Bulina, M. E., Lukyanov, K. A., & Lukyanov, S. A.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2001-12-20
- Volume
- 511
- Issue
- 1-3
- Pages
- 11-14
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2076112920
- Cited by
- 161
- References
- 0
- FWCI
- 7.21
- Type
- journal article
- Language
- en
- Open access
- closed
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- Yanushevich, Y. G., Staroverov, D. B., Savitsky, A. P., Fradkov, A. F., Gurskaya, N. G., Bulina, M. E., Lukyanov, K. A., & Lukyanov, S. A. (2002). A strategy for the generation of non‐aggregating mutants of Anthozoa fluorescent proteins. FEBS Letters. https://doi.org/10.1016/s0014-5793(01)03263-x
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