Testosterone inhibits tumor necrosis factor‐α‐induced vascular cell adhesion molecule‐1 expression in human aortic endothelial cells
Abstract
We investigated the effect of testosterone (T) on tumor necrosis factor‐α (TNF‐α)‐induced expression of vascular cell adhesion molecule‐1 (VCAM‐1) in human aortic endothelial cells. Incubation of these cells with T resulted in a dose‐dependent reduction in the expression, with this reduction completely abolished by a selective androgen receptor blocker. Electrophoretic mobility shift assay demonstrated that T inhibited TNF‐α‐induced activation of the transcriptional nuclear factor‐κB, which is critical for the inducible expression of VCAM‐1, probably through the suppression of the nuclear translocation. Our results may suggest an inhibitory effect of T on atherogenesis, providing a novel insight into the consideration of the pathogenesis of atherosclerosis.
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In cell culture studies testosterone reduces expression of pro-inflammatory cytokines such as TNFa, interleukin 1, and interleukin 6 in human vascular endothelium, monocytes, and fibroblasts.
“In cell culture studies testosterone has been found to reduce the expression of pro-inflammatory cytokines such as TNFa, interleukin 1, and interleukin 6 in human vascular endothelium, monocytes, and fibroblasts.[36][37][38][39]”
From Testosterone replacement in hypogonadal men with angina improves ischaemic threshold and quality of life (2004) · cites this paper as [36]
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References 29 · Cited by 103
- Authors
- Hatakeyama, H., Nishizawa, M., Nakagawa, A., Nakano, S., Kigoshi, T., & Uchida, K.
- Affiliations
- Kanazawa Medical University (JP)
- Corresponding author
- Hatakeyama, Haruhiko
- ORCID
- Nakano, Shigeru
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2002-09-28
- Volume
- 530
- Issue
- 1-3
- Pages
- 129-132
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2057523407
- Cited by
- 103
- References
- 29
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 4 · 2022: 2 · 2021: 4 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 4 · 2015: 4 · 2014: 4 · 2013: 4 · 2012: 10
- FWCI
- 1.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Keywords
- testosterone, TNF-α, VCAM-1, human aortic endothelial cells, androgen receptor, NF-κB activation, nuclear translocation, atherogenesis, atherosclerosis, gene expression regulation
- MeSH terms
- Aorta, Base Sequence, Cells, Cultured, Endothelium, Vascular, Humans, Receptors, Androgen, RNA, Messenger, Testosterone, Blotting, Northern, NF-kappa B, DNA Primers, Vascular Cell Adhesion Molecule-1, Reverse Transcriptase Polymerase Chain Reaction, Electrophoretic Mobility Shift Assay
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