Effect of oxygen on activation state of complex I and lack of oxaloacetate inhibition of complex II in Langendorff perfused rat heart
Open accessAbstract
Two main entry points for electrons into the mitochondrial respiratory chain are NADH:ubiquinone oxidoreductase (complex I) and succinate:ubiquinone oxidoreductase (complex II). Metabolic regulation of these two respiratory complexes is not understood in detail. It has been suggested that the Krebs cycle metabolic intermediate oxaloacetate (OAA) inhibits complex II in vivo, whereas complex I undergoes a reversible active/de‐active transition. In normoxic and anoxic hearts it has been shown that the proportion of complex I in the active and de‐active states is different suggesting a possible mode of regulation of the enzyme by oxygen concentration. In the current studies rapid isolation of mitochondrial membranes in a state that preserves the activity of both complex I and complex II has been achieved using Langendorff perfused rat hearts. The findings indicate that the state of activation of complex I is controlled by the oxygen saturation in the perfusate. In addition, these studies show that complex II is fully active in the mitochondrion and not inhibited by OAA regardless of the oxygen concentration.
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Experimental evidence for oxaloacetate-dependent inhibition of complex II under anoxic conditions is lacking.
“However, experimental evidence for an oxaloacetate-dependent inhibition of complex II under anoxic conditions is lacking [109].”
From Differential effects of complex II on mitochondrial ROS production and their relation to cardioprotective pre- and postconditioning (2013) · cites this paper as [109]
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References 19 · Cited by 37
- Authors
- Maklashina, E., Kotlyar, A. B., Karliner, J. S., & Cecchini, G.
- Affiliations
- San Francisco VA Medical Center (US)
- Corresponding author
- Maklashina, Elena
- Cecchini, Gary
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2003-12-04
- Volume
- 556
- Issue
- 1-3
- Pages
- 64-68
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2084670984
- Cited by
- 37
- References
- 19
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 1 · 2021: 4 · 2020: 2 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 5
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL 68738, P01HL068738)
- National Institute of General Medical Sciences (GM61606, R01GM061606)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Pathology and Forensic Medicine › Cardiac Ischemia and Reperfusion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Coenzyme Q10 studies and effects
- Keywords
- complex I, complex II, mitochondrial respiratory chain, oxygen saturation, enzyme activation state, Krebs cycle intermediates, rat heart
- MeSH terms
- Animals, Hypoxia, Fumarates, Heart, Intracellular Membranes, Male, Malonates, Mitochondria, Heart, Multienzyme Complexes, Myocardium, NADH, NADPH Oxidoreductases, Oxygen, Perfusion, Potassium Cyanide, Succinate Dehydrogenase, Rats, Sprague-Dawley, Electron Transport Complex II, Electron Transport Complex I, Rats, Oxaloacetic Acid, In Vitro Techniques
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