High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria

Korshunov, Sergey S.; Skulachev, Vladimir P.; Starkov, Anatoly A.

doi:10.1016/s0014-5793(97)01159-9

High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria

Formation of H 2 O 2 has been studied in rat heart mitochondria, pretreated with H 2 O 2 and aminotriazole to lower their antioxidant capacity. It is shown that the rate of H 2 O 2 formation by mitochondria oxidizing 6 mM succinate is inhibited by a protonophorous uncoupler, ADP and phosphate, ma…