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Cloning and transcriptional regulation of the gene encoding the vacuolar/H+ ATPase B subunit of Dictyostelium discoideum
Open accessAbstract
The main function of vacuolar H+ ATPases in eukaryotic cells is to generate proton and electrochemical gradients across the membrane of inner compartments. We have isolated the gene encoding the B subunit of Dictyostelium discoideum vacuolar H+ ATPase (vatB) and analyzed its transcriptional regulation. The deduced protein comprises 493 amino acids with a calculated molecular mass of 54 874 Da. The predicted protein sequence is highly homologous to previously determined V/H+ ATPase B subunit sequences. The protein is encoded by a single gene in the Dictyostelium genome. The gene is maximally expressed during growth and it decreases during the first hours of development. Gene expression is rapidly enhanced by phagocytosis, but not by fluid‐phase endocytosis. Acidic and alkaline conditions affect vatB gene expression differently.
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Some genes, such as the gene for the vatB subunit of the V-H+ ATPase or for the eIF6 factor, are expressed at higher rates within 60 min of incubation with bacteria compared to axenic medium.
“some genes, such as the gene for the vatB subunit of the V-H+ ATPase or for the eIF6 factor, which is required for ribosomal assembly, are expressed at higher rates within 60 min of incubation with bacteria, when compared to axenic medium [25,26]”
From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [25]
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Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium
Sillo, A., Bloomfield, G., Balest, A., Balbo, A., Pergolizzi, B., Peracino, B., Skelton, J., Ivens, A., & Bozzaro, S. (2008) · BMC Genomics · doi:10.1186/1471-2164-9-291 · Cited by 69
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Metadata
- Authors
- Bracco, E., Peracino, B., Noegel, A. A., & Bozzaro, S.
- Affiliations
- Ospedale San Luigi Gonzaga (IT)
- Max Planck Institute of Biochemistry (DE)
- Corresponding author
- Bozzaro, Salvatore
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1997-12-08
- Volume
- 419
- Issue
- 1
- Pages
- 37-40
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2156360989
- Cited by
- 12
- References
- 0
- Citations by year
- 2018: 1 · 2017: 1 · 2012: 1
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- European Commission
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- vacuolar H+-ATPase, B subunit, Dictyostelium discoideum, transcriptional regulation, gene cloning, phagocytosis, endocytosis, proton gradient, developmental gene expression
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Dictyostelium, Escherichia coli, Proton-Translocating ATPases, Hydrogen-Ion Concentration, Molecular Sequence Data, Phagocytosis, RNA, Messenger, Transcription, Genetic, Vacuoles, Gene Expression Regulation, Enzymologic, RNA, Protozoan, Genes, Protozoan, Sequence Analysis, DNA, Proton Pumps, Gene Expression Regulation, Developmental, Gene Dosage
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