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A chemical equilibrium model for metal adsorption onto bacterial surfaces
Open accessAbstract
This study quantifies metal adsorption onto cell wall surfaces of Bacillus subtilis by applying equilibrium thermodynamics to the specific chemical reactions that occur at the water-bacteria interface. We use acid/base titrations to determine deprotonation constants for the important surface functional groups, and we perform metal-bacteria adsorption experiments, using Cd, Cu, Pb, and Al, to yield site-specific stability constants for the important metal-bacteria surface complexes. The acid/base properties of the cell wall of B. subtilis can best be characterized by invoking three distinct types of surface organic acid functional groups, with pKa values of 4.82 ± 0.14, 6.9 ± 0.5, and 9.4 ± 0.6. These functional groups likely correspond to carboxyl, phosphate, and hydroxyl sites, respectively, that are displayed on the cell wall surface. The results of the metal adsorption experiments indicate that both the carboxyl sites and the phosphate sites contribute to metal uptake. The values of the log stability constants for metal-carboxyl surface complexes range from 3.4 for Cd, 4.2 for Pb, 4.3 for Cu, to 5.0 for Al. These results suggest that the stabilities of the metal-surface complexes are high enough for metal-bacterial interactions to affect metal mobilities in many aqueous systems, and this approach enables quantitative assessment of the effects of bacteria on metal mobilities.
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“the surface complexation modeling (SCM) approach, grounded in equilibrium thermodynamics through mass action expressions, has been extended beyond minerals to successfully model trace metal adsorption onto microbial surfaces (Fein et al., 1997(Fein et al., , 2005)), including marine cyanobacteria (Liu et al., 2015) and anoxygenic photosynthesizers (Martinez et al., 2016).”
From Trace elements at the intersection of marine biological and geochemical evolution (2016) · cites this paper as Fein et al., 1997
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Fein, J. B., Yu, Q., Nam, J., & Yee, N. (2019) · Chemical Geology · doi:10.1016/j.chemgeo.2019.04.026 · Cited by 65
Self-citationEffects of cultivation conditions on acid–base titration properties of Shewanella putrefaciens
Haas, J. R. (2004) · Chemical Geology · doi:10.1016/j.chemgeo.2004.04.022 · Cited by 65
Metadata
- Authors
- Fein, J. B., Daughney, C. J., Yee, N., & Davis, T. A.
- Affiliations
- University of Notre Dame (US)
- McGill University (CA)
- ORCID
- Yee, Nathan
- Venue
- Geochimica et Cosmochimica Acta
- Publisher
- Elsevier BV
- Published
- 1997-08
- Volume
- 61
- Issue
- 16
- Pages
- 3319-3328
- ISSN
- 0016-7037 (ISSN-L)
- OpenAlex
- W2020140234
- Cited by
- 709
- References
- 24
- Citations by year
- 2026: 12 · 2025: 10 · 2024: 10 · 2023: 18 · 2022: 18 · 2021: 13 · 2020: 18 · 2019: 22 · 2018: 31 · 2017: 25 · 2016: 37 · 2015: 34 · 2014: 30 · 2013: 27 · 2012: 31
- FWCI
- 30.04
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Geochimica et Cosmochimica Acta (publishedVersion, cc-by-nc-nd)
- Topics
- Physical Sciences › Environmental Science › Water Science and Technology › Adsorption and biosorption for pollutant removal
- Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
- Physical Sciences › Environmental Science › Water Science and Technology › Heavy Metal Pollution Remediation
- Keywords
- metal adsorption, bacterial cell wall, Bacillus subtilis, chemical equilibrium model, stability constants, deprotonation constants, carboxyl groups, phosphate groups, hydroxyl groups, cadmium, copper, lead, aluminum, metal mobility, acid-base titration
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