The DOI matches its Crossref record.
“Chemical aminoacylation” of tRNA's
Open accessAbstract
Incubation of abbreviated tRNA's (tRNA-C-COH's) with (chemically) preaminoacylated P1, P2-di(adenosine 5'-)diphosphates in the presence of purified RNA ligase effected transfer of an aminoacyladenylate moiety to the 3'-terminus of the abbreviated tRNA's in good yield. Aminoacylated (or misacylated) tRNA's may thus be prepared from fractionated or unfractionated tRNA-C-COH's; each of the five aminoacylated dinucleoside diphosphates tested was utilized as a substrate by RNA ligase. That the resulting "chemically aminoacylated" tRNA's were identical with those prepared by enzymatic aminoacylation was judged by comparison of 1) chromatographic properties on benzolated diethylaminoethyl-cellulose, 2) rates of chemical deacylation, and 3) affinities for elongation factor Tu, as well as 4) the ability of misacylated tRNA's so derived to be deacylated chemically and then reactivated enzymatically with their cognate amino acids.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Adding New Chemistries to the Genetic Code
Liu, C. C., & Schultz, P. G. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.052308.105824 · Cited by 1,725
Flexizymes for genetic code reprogramming
Goto, Y., Katoh, T., & Suga, H. (2011) · Nature Protocols · doi:10.1038/nprot.2011.331 · Cited by 472
A highly flexible tRNA acylation method for non-natural polypeptide synthesis
Murakami, H., Ohta, A., Ashigai, H., & Suga, H. (2006) · Nature Methods · doi:10.1038/nmeth877 · Cited by 468
Is there a common chemical model for life in the universe?
Benner, S. A., Ricardo, A., & Carrigan, M. A. (2004) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2004.10.003 · Cited by 363
Bain, J. D., Switzer, C., Chamberlin, R., & Benner, S. A. (1992) · Nature · doi:10.1038/356537a0 · Cited by 280
Metadata
- Authors
- Hecht, S. M., Alford, B. L., Kuroda, Y., & Kitano, S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1978-07
- Volume
- 253
- Issue
- 13
- Pages
- 4517-4520
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1582006209
- Cited by
- 194
- References
- 0
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: “Chemical aminoacylation” of tRNA's. https://app.cochat.ai/papers/10.1016/s0021-9258(17)30417-9
- The paper
- Hecht, S. M., Alford, B. L., Kuroda, Y., & Kitano, S. (1978). “Chemical aminoacylation” of tRNA's. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)30417-9
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
