Functional neuronal ionotropic glutamate receptors are expressed in the non-neuronal cell line MIN6.
Open accessAbstract
W e report that a non-neuronal cell line, MIN6, derived from insulin-secreting pancreatic p-cells, naturally expresses functional ionotropic glutamate receptors.Electrophysiological recordings show that kainate, a-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and N-methyh-aspartate (NMDA) depolarize single MING cells and evoke inward ionic currents.These agents also increase the intracellular calcium concentration in MING cells.Furthermore, insulin secretion from MING cells is stimulated by kainate, AMPA, and NMDA.The presence of AMPAkainate and NMDA receptor subtypes is confirmed by reverse transcriptase-polymerase chain reaction.These results demonstrate that ionotropic glutamate receptors with properties similar to those in neuronal cells are expressed in a non-neuronal cell line, MIN6.Thus, MIN6 provides a useful and valuable model system for biochemical, pharmacological, and physiological studies of ionotropic glutamate receptors.Glutamate receptors are the major neurotransmitter receptors mediating synaptic transmission in the vertebrate central nervous system.Glutamate receptors play pivotal roles in synaptic plasticity, neuronal development, and some neurodegenerative disorders (1-5).Although glutamate receptors have been studied extensively in the central nervous system, little is known of the glutamate receptors in non-neuronal cells.It has been shown that functional ionotropic glutamate receptors are Ministry of Education, Science, and Culture and from the Ministry of * This study was supported by scientific research
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Glutamate acts as an excitatory neurotransmitter in the pancreas and stimulates electrical activity and insulin secretion in rat islets.
“Glutamate acts as an excitatory neurotransmitter in the pancreas and stimulates electrical activity and insulin secretion in rat islets (220).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [220]
The Abstract (page not available in our copy) reports that glutamate-receptor agonists depolarize MIN6 pancreatic β-cells and stimulate their insulin secretion. It does not establish that glutamate acts as an excitatory neurotransmitter in the pancreas or show these effects in rat islets; the study used a mouse-derived cell line. (LLM, conf=0.90)
Experimental — accuracy not yet measuredGrin1 is expressed in mouse β-cells and NMDA-activated currents have been recorded in mouse insulinoma cells (MIN6).
“Grin1 is expressed in mouse β-cells (Appendix Figure 14B) and NMDA-activated currents have been recorded in mouse insulinoma cells (MIN6) (220).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [220]
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- Seino, S., Chen, L., Seino, M., Blondel, O., Takeda, J., Johnson, J. H. &Bell, G. I. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 584-588
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Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
- Authors
- Gonoi, T., Mizuno, N., Inagaki, N., Kuromi, H., Seino, Y., Miyazaki, J., & Seino, S.
- Affiliations
- Chiba University (JP)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1994-06
- Volume
- 269
- Issue
- 25
- Pages
- 16989-16992
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1606045266
- Cited by
- 110
- References
- 0
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 6 · 2019: 3 · 2018: 3 · 2017: 6 · 2016: 1 · 2015: 7 · 2014: 2 · 2013: 2 · 2012: 10
- FWCI
- 1.29
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Takeda Science Foundation
- Uehara Memorial Foundation
- Otsuka Pharmaceutical
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- ionotropic glutamate receptors, MIN6 cells, pancreatic beta cells, non-neuronal cells, kainate receptors, AMPA receptors, NMDA receptor, insulin secretion, intracellular calcium, receptor subtypes, glutamate signaling
- MeSH terms
- Insulin Secretion, Animals, Base Sequence, Calcium, Cell Line, Insulin, Islets of Langerhans, Membrane Potentials, Molecular Sequence Data, RNA, Messenger, Signal Transduction, Gene Expression, Receptors, N-Methyl-D-Aspartate, Receptors, Glutamate, DNA Primers, Kainic Acid Receptors, Receptors, Kainic Acid, Mice
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