The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A novel cDNA restores reduced folate carrier activity and methotrexate sensitivity to transport deficient cells.
Open accessAbstract
Mammalian cells accumulate reduced folates and methotrexate, a folate antagonist, through the reduced-folate carrier (RFC) (Goldman, I.D., Lichtenstein, N.S., and Oliverio, V.T. (1968) J. Biol. Chem. 243, 5007-5017). This study describes the isolation and expression of a cDNA clone that restores RFC activity to human breast cancer cells defective in this transporter. The cDNA cancer cells defective in this transporter. The cDNA codes for a peptide (mRFC1) of 58 kDa, whose hydropathy plot, resembling those of mammalian sugar transporters, predicts that it may be a member of a superfamily of transporter genes. Transfection of methotrexate-resistant (MTXR) ZR-75-1 cells with an expression vector, pRFC1, that codes for this peptide restores their ability to accumulate methotrexate. Furthermore, transport of methotrexate into pRFC1-transfected cells is blocked by a 10-fold molar excess of the reduced folate, 5-formyltetrahydrofolic acid, but is unaffected by folic acid. The increase in methotrexate uptake that is observed in pRFC1-transfected MTXR ZR-75-1 cells reverses their resistance to this antitumor agent.
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Cites
Stoscheck, C. M. (1990) · Methods in Enzymology · doi:10.1016/0076-6879(90)82008-p · Cited by 1,438
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Gonzalez-Angulo, A. M., Morales-Vasquez, F., & Hortobagyi, G. N. (2007) · Advances in Experimental Medicine and Biology · doi:10.1007/978-0-387-74039-3_1 · Cited by 904
Antifolates in cancer therapy: Structure, activity and mechanisms of drug resistance
Gonen, N., & Assaraf, Y. G. (2012) · Drug Resistance Updates · doi:10.1016/j.drup.2012.07.002 · Cited by 437
Intestinal absorption of water-soluble vitamins in health and disease
Said, H. M. (2011) · Biochemical Journal · doi:10.1042/bj20110326 · Cited by 419
Matherly, L. H., & Goldman, I. D. (2003) · Vitamins & Hormones · doi:10.1016/s0083-6729(03)01012-4 · Cited by 386
Abe, T., Unno, M., Onogawa, T., Tokui, T., Kondo, T. N., Nakagomi, R., Adachi, H., Fujiwara, K., Okabe, M., Suzuki, T., et al. (2001) · Gastroenterology · doi:10.1053/gast.2001.24804 · Cited by 345
Metadata
- Authors
- Dixon, K. H., Lanpher, B. C., Chiu, J., Kelley, K., & Cowan, K. H.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1994-01
- Volume
- 269
- Issue
- 1
- Pages
- 17-20
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1529220262
- Cited by
- 240
- References
- 1
- FWCI
- 5.20
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Dixon, K. H., Lanpher, B. C., Chiu, J., Kelley, K., & Cowan, K. H. (1994). A novel cDNA restores reduced folate carrier activity and methotrexate sensitivity to transport deficient cells. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)42301-5
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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