The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit.
Open accessAbstract
A fragment of the Drosophila melanogaster RpIIC4 locus, which encodes the RNA polymerase II subunit that determines amanitin sensitivity, was inserted into a bacterial plasmid cloning vehicle useful for over-production of hybrid proteins. Two plasmid constructions encoded hybrid proteins that reacted with antibodies against D. melanogaster RNA polymerase II. Use of subunit-specific antibodies indicated that these hybrid proteins displayed antigenic determinants unique to the largest polypeptide (215 kDa) of the enzyme. This RpII locus, the site at which mutations to amanitin-resistance occur, must therefore encode the largest polymerase II subunit.
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“These mutations, mostly located in the largest RNA Pol II subunit RPB1 (Greenleaf, 1983), which either slow down or accelerate transcription, have allowed huge progress in the characterization of the role of RNA Pol II speed in the regulation of gene expression.”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Greenleaf, 1983
Citations
Cites
Molecular Cloning. A Laboratory Manual
Wood, E. (1983) · Biochemical Education · doi:10.1016/0307-4412(83)90068-7 · Cited by 29,533
A reliable method for the recovery of DNA fragments from agarose and acrylamide gels
Dretzen, G., Gross-Bellard, M., Sassone‐Corsi, P., & Chambon, P. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90296-7 · Cited by 873
Helfman, D. M., Feramisco, J. R., Fiddes, J. C., Thomas, G. P., & Hughes, S. H. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.1.31 · Cited by 302
Guarente, L. (1980) · Cell · doi:10.1016/0092-8674(80)90640-6 · Cited by 226
Searles, L. L., Jokers, R. S., Bingham, P. M., et al. (1982) · Cell · doi:10.1016/0092-8674(82)90314-2 · Cited by 132
α-amanitin-resistant D. melanogaster with an altered RNA polymerase II
Greenleaf, A. L., Borsett, L. M., Jiamachello, P. F., et al. (1979) · Cell · doi:10.1016/0092-8674(79)90116-8 · Cited by 105
Self-citationGenetic and biochemical characterization of mutants at an RNA polymerase II locus in D. melanogaster
Greenleaf, A. L. (1980) · Cell · doi:10.1016/0092-8674(80)90441-9 · Cited by 101
Self-citationWeeks, J. R., Coulter, D. E., & Greenleaf, A. L. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)83862-0 · Cited by 89
Self-citationBrodner, O. G., & Wieland, T. (1976) · Biochemistry · doi:10.1021/bi00661a013 · Cited by 72
Positive-selection cloning vehicle useful for overproduction of hybrid proteins
Cheng, S. C., & Modrich, P. L. (1983) · Journal of Bacteriology · doi:10.1128/jb.154.2.1005-1008.1983 · Cited by 53
Research works printed but not linked: 2
- Cochet-Meilhac, 1974, Biochem. Biophys. Acta, 353, 160
- Davis, 1980, Methods Enzymol., 65, 407
Cited by
RNA polymerase II speed: a key player in controlling and adapting transcriptome composition
Muniz, L., Nicolas, E., & Trouche, D. (2021) · The EMBO Journal · doi:10.15252/embj.2020105740 · Cited by 198
“Conversely, the E1126G substitution in human RPB1 (Fong et al , 2014) (homologous to the E1103G substitution in the yeast RPB1; Kaplan et al , 2008; Kireeva et al , 2008) accelerates transcription by stabilizing a triple alpha helix at the base of the trigger loop, concomitantly with a loss of fidelity that is reduced…”
Metadata
- Authors
- Greenleaf, A. L.
- Affiliations
- Duke Medical Center (US)
- Corresponding author
- Greenleaf, A L
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1983-11
- Volume
- 258
- Issue
- 22
- Pages
- 13403-13406
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1488181583
- Cited by
- 83
- References
- 13
- Citations by year
- 2024: 1 · 2023: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2013: 1 · 2012: 1
- FWCI
- 4.13
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Zenodo (CERN European Organization for Nuclear Research) (cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- RNA polymerase II, RPB1, Drosophila melanogaster, hybrid proteins, antigenic determinants, plasmid cloning
- MeSH terms
- Amanitins, Animals, Drosophila melanogaster, Genes, Molecular Weight, Mutation, Plasmids, RNA Polymerase II, Macromolecular Substances, Protein Multimerization
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