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Kinetics of internalization and recycling of transferrin and the transferrin receptor in a human hepatoma cell line. Effect of lysosomotropic agents

Ciechanover, A, Schwartz, A L, Dautry-Varsat, A, Lodish, H F·1983·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(17)44551-0
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Abstract

Growing HepG2 cells contain 50,000 functional surface transferrin-binding sites (Ciechanover, A., Schwartz, A.L., and Lodish, H.F. (1983) Cell 32,267-275) and 100,000 intracellular sites. At saturating concentrations of [59Fe]transferrin, and under conditions in which protein synthesis is blocked, iron uptake is linear for several hours at a rate of 9,500 transferrin molecules/cell/min. Thus, each receptor must recycle a ligand, on the average, each 15.8 min. Surface-bound transferrin is rapidly endocytosed (t1/2 = 3.5 min). All of the iron remains within the cell, while the apotransferrin is rapidly (t1/2 = 5.0 min) secreted into the medium. Previously, we showed (Dautry-Varsat, A., Ciechanover, A., and Lodish, H.F. (1983) Proc. Natl. Acad. Sci. U.S.A. 80, 2258-2262) that exposure of a ferrotransferrin-receptor complex to medium of pH less than 5.0 results in dissociation of iron, but that apotransferrin remains bound to its receptor. If the pH is raised to 7.0, such as would occur when an acidic intracellular vesicle fuses with the plasma membrane, apotransferrin is very rapidly dissociated (t1/2 = 17 s at 37 degrees C) from its receptor. Taken together, these results indicate that transferrin remains bound to its receptor throughout the endocytic cycle. In the present study, we have directly measured all the kinetic parameters involved in the transferrin receptor cycle. They are similar to those of the asialoglycoprotein receptor in the same cell line, and can be described…

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Research works printed but not linked: 29

  1. Geuze, 1983, Cell, 32, 277, 10.1016/0092-8674(83)90518-4 · DOI
  2. Tycko, 1982, Cell, 28, 643, 10.1016/0092-8674(82)90219-7 · DOI
  3. Klausner, 1983, Proc. Natl. Acad. Sci. U. S. A., 80, 2263, 10.1073/pnas.80.8.2263 · DOI
  4. Tanabe, 1979, J. Biol. Chem., 254, 1038, 10.1016/S0021-9258(17)34164-9 · DOI
  5. Schwartz, 1982, J. Biol. Chem., 257, 4230, 10.1016/S0021-9258(18)34710-0 · DOI
  6. Ciechanover, 1983, Cell, 32, 267, 10.1016/0092-8674(83)90517-2 · DOI
  7. Gonzalez-Noriega, 1980, J. Cell Biol., 85, 839, 10.1083/jcb.85.3.839 · DOI
  8. Stahl, 1980, Cell, 19, 207, 10.1016/0092-8674(80)90402-X · DOI
  9. Brown, 1982, Cold Spring Harbor Symp. Quant. Biol., 46, 713, 10.1101/SQB.1982.046.01.068 · DOI
  10. Van Leuven, 1980, Cell, 20, 37, 10.1016/0092-8674(80)90232-9 · DOI
  11. Marshall, 1981, J. Biol. Chem., 256, 11464, 10.1016/S0021-9258(19)68423-1 · DOI
  12. Jandl, 1963, J. Clin. Invest., 42, 314, 10.1172/JCI104718 · DOI
  13. Karin, 1981, J. Biol. Chem., 256, 3245, 10.1016/S0021-9258(19)69597-9 · DOI
  14. Klausner, 1983, J. Biol. Chem., 258, 4715, 10.1016/S0021-9258(18)32481-5 · DOI
  15. Dautry-Varsat, 1983, Proc. Natl. Acad. Sci. U. S. A., 80, 2258, 10.1073/pnas.80.8.2258 · DOI
  16. Maxfield, 1982, J. Cell Biol., 95, 676, 10.1083/jcb.95.2.676 · DOI
  17. Harford, 1983, J. Biol. Chem., 258, 3191, 10.1016/S0021-9258(18)32848-5 · DOI
  18. Knowles, 1980, Science (Wash. D. C.), 209, 497, 10.1126/science.6248960 · DOI
  19. Schwartz, 1981, J. Biol. Chem., 256, 8878, 10.1016/S0021-9258(19)52477-2 · DOI
  20. Lowry, 1951, J. Biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6 · DOI
  21. Wiley, 1981, Cell, 25, 433, 10.1016/0092-8674(81)90061-1 · DOI
  22. Ward, 1982, J. Biol. Chem., 257, 10317, 10.1016/S0021-9258(18)34022-5 · DOI
  23. van Renswoude, 1982, Proc. Natl. Acad. Sci. U. S. A., 79, 6186, 10.1073/pnas.79.20.6186 · DOI
  24. Ohkuma, 1978, Proc. Natl. Acad. Sci. U. S. A., 75, 3327, 10.1073/pnas.75.7.3327 · DOI
  25. Carpentier, 1982, J. Cell Biol., 95, 73, 10.1083/jcb.95.1.73 · DOI
  26. Goldstein, 1974, J. Biol. Chem., 249, 5153, 10.1016/S0021-9258(19)42341-7 · DOI · Paper page
  27. Octave, 1982, Eur. J. Biochem., 123, 235, 10.1111/j.1432-1033.1982.tb19758.x · DOI
  28. Tolleshaug, 1979, Biochem. Pharmacol., 28, 2919, 10.1016/0006-2952(79)90586-0 · DOI
  29. Tietze, 1980, Biochem. Biophys. Res. Commun., 93, 1, 10.1016/S0006-291X(80)80237-3 · DOI

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Metadata

Authors
Ciechanover, A., Schwartz, A. L., Dautry-Varsat, A., & Lodish, H. F.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1983-08
Volume
258
Issue
16
Pages
9681-9689
ISSN
0021-9258
DOI
10.1016/s0021-9258(17)44551-0
Cited by
434
References
0
Language
en
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