Transcriptional regulation of the tryptophan oxygenase gene in rat liver by glucocorticoids.
Open accessAbstract
The enzyme tryptophan oxygenase (EC 1.13.11.11), which is synthesized in rat liver, is induced by glucocorticoids. We have used cloned tryptophan oxygenase genomic and cDNA sequences to study the mechanism of induction. Rat liver poly(A+) RNA was separated on a formaldehyde gel, blotted to nitrocellulose, and hybridized to a nick-translated tryptophan oxygenase cDNA clone to analyze the kinetics of tryptophan oxygenase mRNA accumulation. Transcription in isolated rat liver nuclei was investigated to determine the relative rate of transcription of the tryptophan oxygenase gene. Analysis of the accumulation of albumin mRNA, which is unaffected by glucocorticoids, served as an internal control. We show here that the synthetic glucocorticoid dexamethasone causes a 10-fold increase in the concentration of tryptophan oxygenase mRNA sequences in rat liver, and that this is a consequence of transcriptional activation of the tryptophan oxygenase gene.
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Hepatic TDO is strongly regulated by glucocorticoids, while IDO is immune responsive.
“Importantly, hepatic TDO is strongly regulated by glucocorticoids (Danesch et al., 1983;Ohta et al., 2017), while IDO is immune responsive.”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Danesch et al., 1983
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References 30 · Cited by 106
- Authors
- Danesch, U., Hashimoto, S., Renkawitz, R., & Schütz, G.
- Affiliations
- German Cancer Research Center (DE)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1983-04
- Volume
- 258
- Issue
- 8
- Pages
- 4750-4753
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1558363775
- Cited by
- 106
- References
- 30
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 2 · 2019: 6 · 2018: 3 · 2017: 3 · 2016: 5 · 2015: 2 · 2014: 2 · 2012: 1
- FWCI
- 6.80
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacogenetics and Drug Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- tryptophan oxygenase, glucocorticoids, dexamethasone, transcriptional regulation, liver, mRNA accumulation, transcriptional activation, rat liver, albumin mRNA
- MeSH terms
- Animals, Dexamethasone, DNA, Glucocorticoids, Kinetics, Liver, Male, Nucleic Acid Hybridization, Poly A, Rats, Inbred Strains, RNA, RNA, Messenger, Transcription, Genetic, Tryptophan Oxygenase, Indoleamine-Pyrrole 2,3,-Dioxygenase, Rats
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