Malolactic enzyme of Lactobacillus plantarum. Purification, properties, and distribution among bacteria.
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The malolactic enzyme of Lactobacillus plantarum was purified from 5.5 units/mg to a specific activity of 265 units/mg of protein. The enzyme has an isoelectric point of pH 4.4. The molecular weight is Mr = 140,000 as determined by gradient gel electrophoresis. The enzyme consists of two probably identical subunits (Mr = 70,000) that were observed after treatment with sodium dodecyl sulfate. Malolactic enzyme catalyzes the NAD- and manganese-dependent reaction L-malate leads to CO2 + L-lactate. Therefore, this enzyme can be distinguished from the well known malic enzymes (L-malate: NAD+ oxidoreductase, oxalacetate-decarboxylating EC 1.1.1.38 or 1.1.1.39). Malolactic enzyme is found in most lactic acid bacteria (Lactobacteriaceae); it has not been detected in other bacteria.
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Malic acid decarboxylation to lactic acid is catalyzed by malate decarboxylase (malolactic enzyme) requiring NAD+ and Mn++.
“This reaction is catalysed by the enzyme malate decarboxylase, often referred to as the malolactic enzyme, with the requirement of cofactors NAD + and Mn ++ (Lonvaud-Funel and Strasser de Saad 1982, Caspritz and Radler 1983, Spettoli et al. 1984, Naori et al. 1990, Kunkee 1991) (Figure 10).”
From Yeast and bacterial modulation of wine aroma and flavour (2005) · cites this paper as Caspritz and Radler 1983
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- Authors
- Caspritz, G., & Radler, F.
- Affiliations
- Johannes Gutenberg University Mainz (DE)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1983-04
- Volume
- 258
- Issue
- 8
- Pages
- 4907-4910
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1660877423
- Cited by
- 146
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 5 · 2018: 6 · 2017: 7 · 2016: 3 · 2015: 1 · 2014: 5 · 2013: 4 · 2012: 2
- FWCI
- 1.08
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Keywords
- Lactobacillus plantarum, enzyme purification, lactic acid bacteria, enzyme subunits, isoelectric point, molecular weight, malic enzyme
- MeSH terms
- Electrophoresis, Polyacrylamide Gel, Isoelectric Point, Lactates, Lactobacillus, Malate Dehydrogenase, Malates, Manganese, Molecular Weight, NAD, Lactic Acid
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