A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: Alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase
Open accessAbstract
A UDP-G1cNAc:a-mannoside p1,6 N-acetylglucosaminyltransferase was identified in cell-free preparations from the mouse lymphoma cell line BW5147.A novel assay employing concanavalin A-Sepharose, lentil lectin-Sepharose, and radioiodinated glycopeptide acceptors was devised to specifically measure the activity of this enzyme.The enzyme transfers N-acetylglucosamine to the C-6 position of a-linked mannose residues and utilizes as acceptors biantennary Asnlinked oligosaccharides containing N-acetylglucosamine, but not sialic acid or galactose, at the nonreducing termini.The product of the enzyme is a triantennary glycopeptide.The level of this enzyme activity was markedly decreased in a mouse lymphoma cell line (BW5147-PHAR2.1),which has previously been shown to be resistant to the cytotoxic effects of the leukoagglutinating lectin (L-PHA) from Phaseolus vulgaris (Trowbridge, I.
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References 21 · Cited by 213
- Authors
- Cummings, R. D., Trowbridge, I. S., & Kornfeld, S.
- Affiliations
- Salk Institute for Biological Studies (US)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1982-11
- Volume
- 257
- Issue
- 22
- Pages
- 13421-13427
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1603050454
- Cited by
- 213
- References
- 21
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 5 · 2023: 2 · 2022: 3 · 2020: 3 · 2019: 3 · 2017: 4 · 2016: 2 · 2015: 4 · 2014: 3 · 2013: 3 · 2012: 3
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
- Funders
- National Cancer Institute (R01CA008759)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Physical Sciences › Chemistry › Organic Chemistry › Carbohydrate Chemistry and Synthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- N-acetylglucosaminyltransferase I, lectin resistance, Phaseolus vulgaris lectin, concanavalin A, lentil lectin, mouse lymphoma cells, glycosylation, N-glycans
- MeSH terms
- Agglutination, Animals, Carbohydrate Conformation, Carbohydrate Sequence, Cell Line, Drug Resistance, Glucosyltransferases, Kinetics, Lymphoma, Neoplasms, Experimental, Substrate Specificity, N-Acetylglucosaminyltransferases, Lectins, Mice
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