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The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme.
Open accessAbstract
Leader (signal) peptidases cleave the NH2-terminal leader sequences of newly synthesized secreted and membrane proteins during, or shortly after, they insert across the membrane. We have constructed a plasmid, pPS9, in which the structural gene for Escherichia coli leader peptidase is under transcriptional control of a lambda promoter, PR. pPS9 also codes for a temperature-sensitive lambda repressor, causing repression of expression of the plasmid leader peptidase gene at 30 degrees C and rapid synthesis of this enzyme at 42 degrees C. Under appropriate growth regimens, leader peptidase is overproduced 75-fold. Leader peptidase from this strain has been purified to homogeneity. It consists of a 37,000-dalton polypeptide which co-migrates with enzyme activity on DEAE-cellulose and chromatofocusing chromatography. Antibody to this leader peptidase is described.
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Citations
Cited by
Multiple Mechanisms of Protein Insertion into and Across Membranes
Wickner, W. T., & Lodish, H. F. (1985) · Science · doi:10.1126/science.4048938 · Cited by 708
Self-citationGrodberg, J., & Dunn, J. J. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.3.1245-1253.1988 · Cited by 676
Lill, R., Dowhan, W., & Wickner, W. (1990) · Cell · doi:10.1016/0092-8674(90)90742-w · Cited by 550
Self-citationBrundage, L., Hendrick, J. P., Schiebel, E., Driessen, A. J. M., & Wickner, W. (1990) · Cell · doi:10.1016/0092-8674(90)90111-q · Cited by 505
Self-citationPaetzel, M., Karla, A., Strynadka, N. C. J., & Dalbey, R. E. (2002) · Chemical Reviews · doi:10.1021/cr010166y · Cited by 440
Metadata
- Authors
- Wolfe, P. B., Silver, P., & Wickner, W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1982-07
- Volume
- 257
- Issue
- 13
- Pages
- 7898-7902
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1541136019
- Cited by
- 177
- References
- 0
- FWCI
- 10.05
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Wolfe, P. B., Silver, P., & Wickner, W. (1982). The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)34466-1
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