The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Members of the Gq alpha subunit gene family activate phospholipase C beta isozymes.
Open accessAbstract
The relative specificities of members of the G alpha q family of GTP-binding proteins were tested for their ability to activate different phosphoinositide-specific phospholipase C (PI-PLC) beta isozymes. Cos-7 cells were transfected with cDNA corresponding to G alpha q, G alpha 11, G alpha 14, and G alpha 16. Most of the recombinant protein was bound to the cell membrane and these membranes were washed to elute endogenous PI-PLC activity. The membrane preparation was reconstituted with purified preparations of the PI-PLC beta isozymes and guanosine 5'-O-thiotriphosphate (GTP gamma S)-stimulated enzyme activity was measured. All four proteins of the G alpha q family were found to stimulate PI-PLC beta 1, with G alpha q and G alpha 11 being most efficient. On the other hand, G alpha 16 was found to most effectively activate PI-PLC beta 2, while G alpha q, G alpha 11, and G alpha 14 showed less stimulation. Specific anti- G alpha 16 antibody blocked the stimulation of both PI-PLC beta 1 and PI-PLC beta 2 in the enriched membrane fraction. We conclude that there is specificity in the interaction of different members of the Gq family with different PI-PLC beta effectors. This specificity may be important in generating tissue- or receptor-specific responses in vivo.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The GTPase superfamily: conserved structure and molecular mechanism
Bourne, H. R., Sanders, D. A., & McCormick, F. (1991) · Nature · doi:10.1038/349117a0 · Cited by 3,392
Type-Specific Regulation of Adenylyl Cyclase by G Protein βγ Subunits
Tang, W.-J., & Gilman, A. G. (1991) · Science · doi:10.1126/science.1962211 · Cited by 1,091
Activation of the β1 isozyme of phospholipase C by α subunits of the Gq class of G proteins
Taylor, S. J., Chae, H. Z., Rhee, S. G., & Exton, J. H. (1991) · Nature · doi:10.1038/350516a0 · Cited by 780
Self-citation
Cited by
Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation
Balla, T. (2013) · Physiological Reviews · doi:10.1152/physrev.00028.2012 · Cited by 1,638
Regulation of Phosphoinositide-Specific Phospholipase C
Rhee, S. G. (2001) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.70.1.281 · Cited by 1,628
Self-citationSmrcka, A. V., & Sternweis, P. C. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)98401-2 · Cited by 458
Mitochondrial calcium exchange in physiology and disease
Garbincius, J. F., & Elrod, J. W. (2022) · Physiological Reviews · doi:10.1152/physrev.00041.2020 · Cited by 412
Thomas, G. M. H., Cunningham, E., Fensome, A., Ball, A., Totty, N. F., Truong, O., Hsuan, J. J., & Cockcroft, S. (1993) · Cell · doi:10.1016/0092-8674(93)90471-2 · Cited by 207
Metadata
- Authors
- Lee, C. H., Park, D., Wu, D., Rhee, S. G., & Simon, M. I.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1992-08
- Volume
- 267
- Issue
- 23
- Pages
- 16044-16047
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1584157616
- Cited by
- 281
- References
- 3
- FWCI
- 11.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Members of the Gq alpha subunit gene family activate phospholipase C beta isozymes. https://app.cochat.ai/papers/10.1016/s0021-9258(18)41962-x
- The paper
- Lee, C. H., Park, D., Wu, D., Rhee, S. G., & Simon, M. I. (1992). Members of the Gq alpha subunit gene family activate phospholipase C beta isozymes. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)41962-x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
