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Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
Open accessAbstract
A method is described for the affinity purification of antibodies using protein samples that have been electrophoretically transferred to diazotized paper. Using differentiated neuroblastoma cells as the protein sample and a heterogeneous anti-microtubule protein serum, antibodies were isolated that specifically bound only to tubulin on blots and that stained microtubule networks in cells. The general utility of this method for various types of applications is discussed.
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Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Cited by
Cole, D. G., Diener, D. R., Himelblau, A. L., Beech, P. L., Fuster, J. C., & Rosenbaum, J. L. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.141.4.993 · Cited by 898
Protein blotting: Principles and applications
Gershoni, J. M., & Palade, G. E. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90128-8 · Cited by 853
Kozminski, K. G., Beech, P. L., & Rosenbaum, J. L. (1995) · The Journal of cell biology · doi:10.1083/jcb.131.6.1517 · Cited by 586
The DHC1b (DHC2) Isoform of Cytoplasmic Dynein Is Required for Flagellar Assembly
Pazour, G. J., Dickert, B. L., & Witman, G. B. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.144.3.473 · Cited by 471
The v-myb oncogene product binds to and activates the promyelocyte-specific mim-1 gene
Ness, S. A., Marknell, Å., & Graf, T. (1989) · Cell · doi:10.1016/0092-8674(89)90767-8 · Cited by 445
Metadata
- Authors
- Olmsted, J. B.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1981-12
- Volume
- 256
- Issue
- 23
- Pages
- 11955-11957
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1566845757
- Cited by
- 802
- References
- 1
- FWCI
- 17.86
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Olmsted, J. B. (1981). Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)43211-5
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
